Modeling and Measuring Signal Relay in Noisy Directed Migration of Cell Groups

We develop a coarse-grained stochastic model for the influence of signal relay on the collective behavior of migrating Dictyostelium discoideum cells. In the experiment, cells display a range of collective migration patterns, including uncorrelated motion, formation of partially localized streams, and clumping, depending on the type of cell and the strength of the external, linear concentration gradient of the signaling molecule cyclic adenosine monophosphate (cAMP). From our model, we find that the pattern of migration can be quantitatively described by the competition of two processes, the secretion rate of cAMP by the cells and the degradation rate of cAMP in the gradient chamber. Model simulations are compared to experiments for a wide range of strengths of an external linear-gradient signal. With degradation, the model secreting cells form streams and efficiently transverse the gradient, but without degradation, we find that model secreting cells form clumps without streaming. This indicates that the observed effective collective migration in streams requires not only signal relay but also degradation of the signal. In addition, our model allows us to detect and quantify precursors of correlated motion, even when cells do not exhibit obvious streaming.Author Summary: Collective cell migration is observed in various biological processes including angiogenesis, gastrulation, fruiting body formation, and wound healing. Dictyostelium discoideum, for example, exhibits highly dynamic patterns such as streams and clumps during its early phases of collective motion and has served as a model organism for the study of collective migration. In this study, facilitated by experiments, we develop a conceptual, minimalistic, computational model to analyze the dynamical processes leading to the emergence of collective patterns and the associated dependence on the external injection of a cAMP signal, the intercellular cAMP secretion rate, and the cAMP degradation rate. We demonstrate that degradation is necessary to reproduce the experimentally observed collective migration patterns, and show how our model can be utilized to uncover basic dependences of migration modes on cell characteristics. Our numerical observations elucidate the different possible types of motion and quantify the onset of collective motion. Thus, the model allows us to distinguish noisy motion guided by the external signal from weakly correlated motion.