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A Quorum-Sensing Factor in Vegetative Dictyostelium Discoideum Cells Revealed by Quantitative Migration Analysis

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  • Laurent Golé
  • Charlotte Rivière
  • Yoshinori Hayakawa
  • Jean-Paul Rieu

Abstract

Background: Many cells communicate through the production of diffusible signaling molecules that accumulate and once a critical concentration has been reached, can activate or repress a number of target genes in a process termed quorum sensing (QS). In the social amoeba Dictyostelium discoideum, QS plays an important role during development. However little is known about its effect on cell migration especially in the growth phase. Methods and Findings: To investigate the role of cell density on cell migration in the growth phase, we use multisite timelapse microscopy and automated cell tracking. This analysis reveals a high heterogeneity within a given cell population, and the necessity to use large data sets to draw reliable conclusions on cell motion. In average, motion is persistent for short periods of time (), but normal diffusive behavior is recovered over longer time periods. The persistence times are positively correlated with the migrated distances. Interestingly, the migrated distance decreases as well with cell density. The adaptation of cell migration to cell density highlights the role of a secreted quorum sensing factor (QSF) on cell migration. Using a simple model describing the balance between the rate of QSF generation and the rate of QSF dilution, we were able to gather all experimental results into a single master curve, showing a sharp cell transition between high and low motile behaviors with increasing QSF. Conclusion: This study unambiguously demonstrates the central role played by QSF on amoeboid motion in the growth phase.

Suggested Citation

  • Laurent Golé & Charlotte Rivière & Yoshinori Hayakawa & Jean-Paul Rieu, 2011. "A Quorum-Sensing Factor in Vegetative Dictyostelium Discoideum Cells Revealed by Quantitative Migration Analysis," PLOS ONE, Public Library of Science, vol. 6(11), pages 1-9, November.
    • Handle: RePEc:plo:pone00:0026901
    DOI: 10.1371/journal.pone.0026901
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    References listed on IDEAS

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    1. Cao, Shan & Wang, Jiadao & Li, Dangguo & Chen, Darong, 2013. "Ecological and social modeling for migration and adhesion pattern of a benthic diatom," Ecological Modelling, Elsevier, vol. 250(C), pages 269-278.
    2. Can Guven & Erin Rericha & Edward Ott & Wolfgang Losert, 2013. "Modeling and Measuring Signal Relay in Noisy Directed Migration of Cell Groups," PLOS Computational Biology, Public Library of Science, vol. 9(5), pages 1-13, May.

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