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A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling

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Listed:
  • Fangming Wu

    (Chinese Academy of Sciences
    Shandong Agricultural University
    University of Chinese Academy of Sciences)

  • Chuanlong Sun

    (Shandong Agricultural University
    Shandong Agricultural University)

  • Ziying Zhu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Lei Deng

    (Shandong Agricultural University
    Shandong Agricultural University)

  • Feifei Yu

    (China Agricultural University)

  • Qi Xie

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Chuanyou Li

    (Chinese Academy of Sciences
    Shandong Agricultural University
    University of Chinese Academy of Sciences
    Shandong Agricultural University)

Abstract

Mediator25 (MED25) has been ascribed as a signal-processing and -integrating center that controls jasmonate (JA)-induced and MYC2-dependent transcriptional output. A better understanding of the regulation of MED25 stability will undoubtedly advance our knowledge of the precise regulation of JA signaling-related transcriptional output. Here, we report that Arabidopsis MED16 activates JA-responsive gene expression by promoting MED25 stability. Conversely, two homologous E3 ubiquitin ligases, MED25-BINDING RING-H2 PROTEIN1 (MBR1) and MBR2, negatively regulate JA-responsive gene expression by promoting MED25 degradation. MED16 competes with MBR1&2 to bind to the von Willebrand Factor A (vWF-A) domain of MED25, thereby antagonizing the MBR1&2-mediated degradation of MED25 in vivo. In addition, we show that MED16 promotes hormone-induced interactions between MYC2 and MED25, leading to the activation of JA-responsive gene expression. Collectively, our findings reveal a multiprotein regulatory module that robustly and tightly maintains MED25 homeostasis, which determines the strength of the transcriptional output of JA signaling.

Suggested Citation

  • Fangming Wu & Chuanlong Sun & Ziying Zhu & Lei Deng & Feifei Yu & Qi Xie & Chuanyou Li, 2025. "A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-56041-3
    DOI: 10.1038/s41467-025-56041-3
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