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Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria

Author

Listed:
  • Xiaoyue Chen

    (University of California
    Joint BioEnergy Institute
    Lawrence Berkeley National Laboratory)

  • Graham A. Hudson

    (Joint BioEnergy Institute
    University of California)

  • Charlotte Mineo

    (University of California
    Joint BioEnergy Institute)

  • Bashar Amer

    (Joint BioEnergy Institute
    Lawrence Berkeley National Laboratory)

  • Edward E. K. Baidoo

    (Joint BioEnergy Institute
    Lawrence Berkeley National Laboratory)

  • Samantha A. Crowe

    (Joint BioEnergy Institute
    University of California
    University of California)

  • Yuzhong Liu

    (Joint BioEnergy Institute
    University of California)

  • Jay D. Keasling

    (Joint BioEnergy Institute
    University of California
    Lawrence Berkeley National Laboratory
    University of California)

  • Henrik V. Scheller

    (University of California
    Joint BioEnergy Institute
    Lawrence Berkeley National Laboratory)

Abstract

Methyl jasmonate (MeJA) is a known elicitor of plant specialized metabolism, including triterpenoid saponins. Saponaria vaccaria is an annual herb used in traditional Chinese medicine, containing large quantities of oleanane-type triterpenoid saponins with anticancer properties and structural similarities to the vaccine adjuvant QS-21. Leveraging the MeJA-elicited saponin biosynthesis, we identify multiple enzymes catalyzing the oxidation and glycosylation of triterpenoids in S. vaccaria. This exploration is aided by Pacbio full-length transcriptome sequencing and gene expression analysis. A cellulose synthase-like enzyme can not only glucuronidate triterpenoid aglycones but also alter the product profile of a cytochrome P450 monooxygenase via preference for the aldehyde intermediate. Furthermore, the discovery of a UDP-glucose 4,6-dehydratase and a UDP-4-keto-6-deoxy-glucose reductase reveals the biosynthetic pathway for the rare nucleotide sugar UDP-d-fucose, a likely sugar donor for fucosylation of plant natural products. Our work enables the production and optimization of high-value saponins in microorganisms and plants through synthetic biology approaches.

Suggested Citation

  • Xiaoyue Chen & Graham A. Hudson & Charlotte Mineo & Bashar Amer & Edward E. K. Baidoo & Samantha A. Crowe & Yuzhong Liu & Jay D. Keasling & Henrik V. Scheller, 2023. "Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42877-0
    DOI: 10.1038/s41467-023-42877-0
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