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Single-cell analyses of polyclonal Plasmodium vivax infections and their consequences on parasite transmission

Author

Listed:
  • Brittany Hazzard

    (University of Maryland School of Medicine)

  • Juliana M. Sá

    (National Institutes of Health)

  • Haikel N. Bogale

    (University of Maryland School of Medicine)

  • Tales V. Pascini

    (National Institutes of Health)

  • Angela C. Ellis

    (National Institutes of Health)

  • Shuchi Amin

    (National Institutes of Health)

  • Jennifer S. Armistead

    (National Institutes of Health
    University of South Florida)

  • John H. Adams

    (University of South Florida)

  • Thomas E. Wellems

    (National Institutes of Health)

  • David Serre

    (University of Maryland School of Medicine
    University of Maryland School of Medicine)

Abstract

Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on the development of asexual parasites, their sexual differentiation, and their transmission remain unknown. We describe infections of Saimiri monkeys with two strains of P. vivax and the analyses of 80,024 parasites characterized by single cell RNA sequencing and individually genotyped. In our model, consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous inoculations of two strains lead to sustained polyclonal infections, although without detectable differences in parasite regulation or sexual commitment. Analyses of sporozoites dissected from mosquitoes fed on coinfected monkeys show that all genotypes are successfully transmitted to mosquitoes. However, after sporozoite inoculation, not all genotypes contribute to the subsequent blood infections, highlighting an important bottleneck during pre-erythrocytic development. Overall, these studies provide new insights on the mechanisms regulating the establishment of polyclonal P. vivax infections and their consequences for disease transmission.

Suggested Citation

  • Brittany Hazzard & Juliana M. Sá & Haikel N. Bogale & Tales V. Pascini & Angela C. Ellis & Shuchi Amin & Jennifer S. Armistead & John H. Adams & Thomas E. Wellems & David Serre, 2024. "Single-cell analyses of polyclonal Plasmodium vivax infections and their consequences on parasite transmission," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51949-8
    DOI: 10.1038/s41467-024-51949-8
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