Author
Listed:
- Eliana Real
(Department of Life Sciences, Imperial College London)
- Virginia M. Howick
(Parasites and Microbes Programme, Wellcome Sanger Institute
University of Glasgow
University of Glasgow)
- Farah A. Dahalan
(Department of Life Sciences, Imperial College London)
- Kathrin Witmer
(Department of Life Sciences, Imperial College London
Parasites and Microbes Programme, Wellcome Sanger Institute)
- Juliana Cudini
(Parasites and Microbes Programme, Wellcome Sanger Institute)
- Clare Andradi-Brown
(Department of Life Sciences, Imperial College London
Department of Infectious Disease, Imperial College London)
- Joshua Blight
(Department of Life Sciences, Imperial College London)
- Mira S. Davidson
(Department of Life Sciences, Imperial College London)
- Sunil Kumar Dogga
(Parasites and Microbes Programme, Wellcome Sanger Institute)
- Adam J. Reid
(Parasites and Microbes Programme, Wellcome Sanger Institute)
- Jake Baum
(Department of Life Sciences, Imperial College London)
- Mara K. N. Lawniczak
(Parasites and Microbes Programme, Wellcome Sanger Institute)
Abstract
Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics to illuminate gene usage across the transmission cycle of the most virulent agent of human malaria - Plasmodium falciparum. We reveal developmental trajectories associated with the colonization of the mosquito midgut and salivary glands and elucidate the transcriptional signatures of each transmissible stage. Additionally, we identify both conserved and non-conserved gene usage between human and rodent parasites, which point to both essential mechanisms in malaria transmission and species-specific adaptations potentially linked to host tropism. Together, the data presented here, which are made freely available via an interactive website, provide a fine-grained atlas that enables intensive investigation of the P. falciparum transcriptional journey. As well as providing insights into gene function across the transmission cycle, the atlas opens the door for identification of drug and vaccine targets to stop malaria transmission and thereby prevent disease.
Suggested Citation
Eliana Real & Virginia M. Howick & Farah A. Dahalan & Kathrin Witmer & Juliana Cudini & Clare Andradi-Brown & Joshua Blight & Mira S. Davidson & Sunil Kumar Dogga & Adam J. Reid & Jake Baum & Mara K. , 2021.
"A single-cell atlas of Plasmodium falciparum transmission through the mosquito,"
Nature Communications, Nature, vol. 12(1), pages 1-13, December.
Handle:
RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23434-z
DOI: 10.1038/s41467-021-23434-z
Download full text from publisher
Citations
Citations are extracted by the
CitEc Project, subscribe to its
RSS feed for this item.
Cited by:
- Nicholas L. Dooley & Tinashe G. Chabikwa & Zuleima Pava & Jessica R. Loughland & Julianne Hamelink & Kiana Berry & Dean Andrew & Megan S. F. Soon & Arya SheelaNair & Kim A. Piera & Timothy William & B, 2023.
"Single cell transcriptomics shows that malaria promotes unique regulatory responses across multiple immune cell subsets,"
Nature Communications, Nature, vol. 14(1), pages 1-22, December.
- Mwikali Kioko & Alena Pance & Shaban Mwangi & David Goulding & Alison Kemp & Martin Rono & Lynette Isabella Ochola-Oyier & Pete C. Bull & Philip Bejon & Julian C. Rayner & Abdirahman I. Abdi, 2023.
"Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum,"
Nature Communications, Nature, vol. 14(1), pages 1-11, December.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23434-z. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.