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Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis

Author

Listed:
  • Mubasher Mohammed

    (Stockholm University)

  • Alexis Dziedziech

    (Stockholm University
    Institut Pasteur)

  • Diego Macedo

    (Stockholm University)

  • Frederik Huppertz

    (Stockholm University)

  • Ylva Veith

    (Stockholm University)

  • Zoé Postel

    (Stockholm University)

  • Elena Christ

    (Stockholm University)

  • Richard Scheytt

    (Stockholm University)

  • Tanja Slotte

    (Stockholm University)

  • Johan Henriksson

    (Umeå University)

  • Johan Ankarklev

    (Stockholm University
    Biomedical Center (BMC) Uppsala University)

Abstract

The Plasmodium falciparum life cycle includes obligate transition between a human and mosquito host. Gametocytes are responsible for transmission from the human to the mosquito vector where gamete fusion followed by meiosis occurs. To elucidate how male and female gametocytes differentiate in the absence of sex chromosomes, we perform FACS-based cell enrichment of a P. falciparum gametocyte reporter line followed by single-cell RNA-seq. In our analyses we define the transcriptional programs and predict candidate driver genes underlying male and female development, including genes from the ApiAP2 family of transcription factors. A motif-driven, gene regulatory network analysis indicates that AP2-G5 specifically modulates male development. Additionally, genes linked to the inner membrane complex, involved in morphological changes, are uniquely expressed in the female lineage. The transcriptional programs of male and female development detailed herein allow for further exploration of the evolution of sex in eukaryotes and provide targets for future development of transmission blocking therapies.

Suggested Citation

  • Mubasher Mohammed & Alexis Dziedziech & Diego Macedo & Frederik Huppertz & Ylva Veith & Zoé Postel & Elena Christ & Richard Scheytt & Tanja Slotte & Johan Henriksson & Johan Ankarklev, 2024. "Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51201-3
    DOI: 10.1038/s41467-024-51201-3
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