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Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission

Author

Listed:
  • Camilla Luiza-Batista

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite
    Sorbonne Université, École Doctorale Complexité du Vivant)

  • Sabine Thiberge

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Malika Serra-Hassoun

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Flore Nardella

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Aurélie Claës

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Vanessa C. Nicolete

    (University of São Paulo)

  • Pierre-Henri Commère

    (Institut Pasteur, Université Paris Cité, Cytometry and Biomarkers UTechS, CRT)

  • Liliana Mancio-Silva

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Marcelo U. Ferreira

    (University of São Paulo)

  • Artur Scherf

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

  • Sylvie Garcia

    (Institut Pasteur, Université Paris Cité, Inserm U1201, CNRS EMR9195, Unité de Biologie des Interactions Hôte-Parasite)

Abstract

Plasmodium vivax is the most widespread human malaria parasite. Due to the presence of extravascular reservoirs and relapsing infections from dormant liver stages, P. vivax is particularly difficult to control and eliminate. Experimental research is hampered by the inability to maintain P. vivax cultures in vitro, due to its tropism for immature red blood cells (RBCs). Here, we describe a new humanized mice model that can support efficient human erythropoiesis and maintain long-lasting multiplication of inoculated cryopreserved P. vivax parasites and their sexual differentiation, including in bone marrow. Mature gametocytes were transmitted to Anopheles mosquitoes, which led to the formation of salivary gland sporozoites. Importantly, blood-stage P. vivax parasites were maintained after the secondary transfer of fresh or frozen infected bone marrow cells to naïve chimeras. This model provides a unique tool for investigating, in vivo, the biology of intraerythrocytic P. vivax.

Suggested Citation

  • Camilla Luiza-Batista & Sabine Thiberge & Malika Serra-Hassoun & Flore Nardella & Aurélie Claës & Vanessa C. Nicolete & Pierre-Henri Commère & Liliana Mancio-Silva & Marcelo U. Ferreira & Artur Scherf, 2022. "Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31864-6
    DOI: 10.1038/s41467-022-31864-6
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    Cited by:

    1. Brittany Hazzard & Juliana M. Sá & Haikel N. Bogale & Tales V. Pascini & Angela C. Ellis & Shuchi Amin & Jennifer S. Armistead & John H. Adams & Thomas E. Wellems & David Serre, 2024. "Single-cell analyses of polyclonal Plasmodium vivax infections and their consequences on parasite transmission," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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