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Structural insights into strigolactone catabolism by carboxylesterases reveal a conserved conformational regulation

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  • Malathy Palayam

    (University of California—Davis)

  • Linyi Yan

    (University of California—Davis)

  • Ugrappa Nagalakshmi

    (University of California—Davis)

  • Amelia K. Gilio

    (University of California—Davis)

  • David Cornu

    (Université Paris-Saclay, CEA, CNRS)

  • François-Didier Boyer

    (Université Paris-Saclay, CNRS UPR 2301)

  • Savithramma P. Dinesh-Kumar

    (University of California—Davis
    University of California—Davis)

  • Nitzan Shabek

    (University of California—Davis)

Abstract

Phytohormone levels are regulated through specialized enzymes, participating not only in their biosynthesis but also in post-signaling processes for signal inactivation and cue depletion. Arabidopsis thaliana (At) carboxylesterase 15 (CXE15) and carboxylesterase 20 (CXE20) have been shown to deplete strigolactones (SLs) that coordinate various growth and developmental processes and function as signaling molecules in the rhizosphere. Here, we elucidate the X-ray crystal structures of AtCXE15 (both apo and SL intermediate bound) and AtCXE20, revealing insights into the mechanisms of SL binding and catabolism. The N-terminal regions of CXE15 and CXE20 exhibit distinct secondary structures, with CXE15 characterized by an alpha helix and CXE20 by an alpha/beta fold. These structural differences play pivotal roles in regulating variable SL hydrolysis rates. Our findings, both in vitro and in planta, indicate that a transition of the N-terminal helix domain of CXE15 between open and closed forms facilitates robust SL hydrolysis. The results not only illuminate the distinctive process of phytohormone breakdown but also uncover a molecular architecture and mode of plasticity within a specific class of carboxylesterases.

Suggested Citation

  • Malathy Palayam & Linyi Yan & Ugrappa Nagalakshmi & Amelia K. Gilio & David Cornu & François-Didier Boyer & Savithramma P. Dinesh-Kumar & Nitzan Shabek, 2024. "Structural insights into strigolactone catabolism by carboxylesterases reveal a conserved conformational regulation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50928-3
    DOI: 10.1038/s41467-024-50928-3
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