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The main oxidative inactivation pathway of the plant hormone auxin

Author

Listed:
  • Ken-ichiro Hayashi

    (Okayama University of Science)

  • Kazushi Arai

    (Okayama University of Science)

  • Yuki Aoi

    (United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology)

  • Yuka Tanaka

    (Okayama University of Science)

  • Hayao Hira

    (Graduate School of Agriculture, Tokyo University of Agriculture and Technology)

  • Ruipan Guo

    (Section of Cell and Developmental Biology, University of California San Diego)

  • Yun Hu

    (Section of Cell and Developmental Biology, University of California San Diego)

  • Chennan Ge

    (Section of Cell and Developmental Biology, University of California San Diego)

  • Yunde Zhao

    (Section of Cell and Developmental Biology, University of California San Diego)

  • Hiroyuki Kasahara

    (Graduate School of Agriculture, Tokyo University of Agriculture and Technology
    RIKEN Center for Sustainable Resource Science)

  • Kosuke Fukui

    (Okayama University of Science)

Abstract

Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development.

Suggested Citation

  • Ken-ichiro Hayashi & Kazushi Arai & Yuki Aoi & Yuka Tanaka & Hayao Hira & Ruipan Guo & Yun Hu & Chennan Ge & Yunde Zhao & Hiroyuki Kasahara & Kosuke Fukui, 2021. "The main oxidative inactivation pathway of the plant hormone auxin," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27020-1
    DOI: 10.1038/s41467-021-27020-1
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    References listed on IDEAS

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    1. Sayaka Takehara & Shun Sakuraba & Bunzo Mikami & Hideki Yoshida & Hisako Yoshimura & Aya Itoh & Masaki Endo & Nobuhisa Watanabe & Takayuki Nagae & Makoto Matsuoka & Miyako Ueguchi-Tanaka, 2020. "A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
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    1. Malathy Palayam & Linyi Yan & Ugrappa Nagalakshmi & Amelia K. Gilio & David Cornu & François-Didier Boyer & Savithramma P. Dinesh-Kumar & Nitzan Shabek, 2024. "Structural insights into strigolactone catabolism by carboxylesterases reveal a conserved conformational regulation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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