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Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure

Author

Listed:
  • Jia Zhou

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Qinli Hu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xinlong Xiao

    (Chinese Academy of Sciences)

  • Deqiang Yao

    (Shanghai Jiao Tong University School of Medicine)

  • Shenghong Ge

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jin Ye

    (University of Science and Technology of China)

  • Haojie Li

    (Chinese Academy of Sciences)

  • Rujie Cai

    (Shanghai Normal University)

  • Renyang Liu

    (Chinese Academy of Sciences)

  • Fangang Meng

    (Capital Medical University)

  • Chao Wang

    (University of Science and Technology of China)

  • Jian-Kang Zhu

    (Chinese Academy of Sciences)

  • Mingguang Lei

    (Chinese Academy of Sciences)

  • Weiman Xing

    (Shanghai Normal University)

Abstract

Phosphate, a key plant nutrient, is perceived through inositol polyphosphates (InsPs) by SPX domain-containing proteins. SPX1 an inhibit the PHR2 transcription factor to maintain Pi homeostasis. How SPX1 recognizes an InsP molecule and represses transcription activation by PHR2 remains unclear. Here we show that, upon binding InsP6, SPX1 can disrupt PHR2 dimers and form a 1:1 SPX1-PHR2 complex. The complex structure reveals that SPX1 helix α1 can impose a steric hindrance when interacting with the PHR2 dimer. By stabilizing helix α1, InsP6 allosterically decouples the PHR2 dimer and stabilizes the SPX1-PHR2 interaction. In doing so, InsP6 further allows SPX1 to engage with the PHR2 MYB domain and sterically block its interaction with DNA. Taken together, our results suggest that, upon sensing the surrogate signals of phosphate, SPX1 inhibits PHR2 via a dual mechanism that attenuates dimerization and DNA binding activities of PHR2.

Suggested Citation

  • Jia Zhou & Qinli Hu & Xinlong Xiao & Deqiang Yao & Shenghong Ge & Jin Ye & Haojie Li & Rujie Cai & Renyang Liu & Fangang Meng & Chao Wang & Jian-Kang Zhu & Mingguang Lei & Weiman Xing, 2021. "Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27391-5
    DOI: 10.1038/s41467-021-27391-5
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    References listed on IDEAS

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    1. Zeyuan Guan & Juan Chen & Ruiwen Liu & Yanke Chen & Qiong Xing & Zhangmeng Du & Meng Cheng & Jianjian Hu & Wenhui Zhang & Wencong Mei & Beijing Wan & Qiang Wang & Jie Zhang & Peng Cheng & Huanyu Cai &, 2023. "The cytoplasmic synthesis and coupled membrane translocation of eukaryotic polyphosphate by signal-activated VTC complex," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Yukari Nagatoshi & Kenta Ikazaki & Yasufumi Kobayashi & Nobuyuki Mizuno & Ryohei Sugita & Yumiko Takebayashi & Mikiko Kojima & Hitoshi Sakakibara & Natsuko I. Kobayashi & Keitaro Tanoi & Kenichiro Fuj, 2023. "Phosphate starvation response precedes abscisic acid response under progressive mild drought in plants," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Zeyuan Guan & Qunxia Zhang & Zhifei Zhang & Jiaqi Zuo & Juan Chen & Ruiwen Liu & Julie Savarin & Larissa Broger & Peng Cheng & Qiang Wang & Kai Pei & Delin Zhang & Tingting Zou & Junjie Yan & Ping Yin, 2022. "Mechanistic insights into the regulation of plant phosphate homeostasis by the rice SPX2 – PHR2 complex," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    4. Li Lin Xu & Meng Qi Cui & Chen Xu & Miao Jing Zhang & Gui Xin Li & Ji Ming Xu & Xiao Dan Wu & Chuan Zao Mao & Wo Na Ding & Moussa Benhamed & Zhong Jie Ding & Shao Jian Zheng, 2024. "A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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