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Strigolactone perception and deactivation by a hydrolase receptor DWARF14

Author

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  • Yoshiya Seto

    (Tohoku University
    RIKEN Plant Science Center, Tsurumi
    Salk Institute for Biological Studies
    Salk Institute for Biological Studies)

  • Rei Yasui

    (Tohoku University)

  • Hiromu Kameoka

    (University of Tokyo, Yayoi, Bunkyo
    Osaka Prefecture University)

  • Muluneh Tamiru

    (Iwate Biotechnology Research Center
    La Trobe University)

  • Mengmeng Cao

    (Tohoku University)

  • Ryohei Terauchi

    (Iwate Biotechnology Research Center
    Kyoto University)

  • Akane Sakurada

    (Tohoku University)

  • Rena Hirano

    (Tohoku University)

  • Takaya Kisugi

    (Tohoku University)

  • Atsushi Hanada

    (Tohoku University
    RIKEN Plant Science Center, Tsurumi)

  • Mikihisa Umehara

    (RIKEN Plant Science Center, Tsurumi
    Toyo University)

  • Eunjoo Seo

    (RIKEN Plant Science Center, Tsurumi)

  • Kohki Akiyama

    (Osaka Prefecture University)

  • Jason Burke

    (Salk Institute for Biological Studies
    Salk Institute for Biological Studies)

  • Noriko Takeda-Kamiya

    (RIKEN Plant Science Center, Tsurumi)

  • Weiqiang Li

    (RIKEN Plant Science Center, Tsurumi
    RIKEN Center for Sustainable Resource Science)

  • Yoshinori Hirano

    (Nara Institute of Science and Technology)

  • Toshio Hakoshima

    (Nara Institute of Science and Technology)

  • Kiyoshi Mashiguchi

    (Tohoku University)

  • Joseph P. Noel

    (Salk Institute for Biological Studies
    Salk Institute for Biological Studies)

  • Junko Kyozuka

    (Tohoku University
    University of Tokyo, Yayoi, Bunkyo)

  • Shinjiro Yamaguchi

    (Tohoku University
    RIKEN Plant Science Center, Tsurumi
    Kyoto University)

Abstract

The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.

Suggested Citation

  • Yoshiya Seto & Rei Yasui & Hiromu Kameoka & Muluneh Tamiru & Mengmeng Cao & Ryohei Terauchi & Akane Sakurada & Rena Hirano & Takaya Kisugi & Atsushi Hanada & Mikihisa Umehara & Eunjoo Seo & Kohki Akiy, 2019. "Strigolactone perception and deactivation by a hydrolase receptor DWARF14," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08124-7
    DOI: 10.1038/s41467-018-08124-7
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    Cited by:

    1. Dawei Wang & Zhili Pang & Haiyang Yu & Benjamin Thiombiano & Aimee Walmsley & Shuyi Yu & Yingying Zhang & Tao Wei & Lu Liang & Jing Wang & Xin Wen & Harro J. Bouwmeester & Ruifeng Yao & Zhen Xi, 2022. "Probing strigolactone perception mechanisms with rationally designed small-molecule agonists stimulating germination of root parasitic weeds," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Malathy Palayam & Linyi Yan & Ugrappa Nagalakshmi & Amelia K. Gilio & David Cornu & François-Didier Boyer & Savithramma P. Dinesh-Kumar & Nitzan Shabek, 2024. "Structural insights into strigolactone catabolism by carboxylesterases reveal a conserved conformational regulation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Kyoichi Kodama & Mélanie K. Rich & Akiyoshi Yoda & Shota Shimazaki & Xiaonan Xie & Kohki Akiyama & Yohei Mizuno & Aino Komatsu & Yi Luo & Hidemasa Suzuki & Hiromu Kameoka & Cyril Libourel & Jean Kelle, 2022. "An ancestral function of strigolactones as symbiotic rhizosphere signals," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Satoshi Ogawa & Songkui Cui & Alexandra R. F. White & David C. Nelson & Satoko Yoshida & Ken Shirasu, 2022. "Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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