IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30771-0.html
   My bibliography  Save this article

Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana

Author

Listed:
  • Si Liu

    (University of California)

  • Meijuan Chen

    (University of California)

  • Ruidong Li

    (University of California)

  • Wan-Xiang Li

    (University of California)

  • Amit Gal-On

    (Volcani Center)

  • Zhenyu Jia

    (University of California)

  • Shou-Wei Ding

    (University of California)

Abstract

Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant virus resistance are enriched in genes of RNA interference (RNAi) pathway known to confer essential antiviral defense in plants. Here, we report two genome-wide association study screens to interrogate natural variation among wild-collected Arabidopsis thaliana accessions in quantitative resistance to the endemic cucumber mosaic virus (CMV). We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0. One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs). Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance. Our findings identify positive and negative regulators of the antiviral RNAi defense that may play important roles in virus-host coevolution.

Suggested Citation

  • Si Liu & Meijuan Chen & Ruidong Li & Wan-Xiang Li & Amit Gal-On & Zhenyu Jia & Shou-Wei Ding, 2022. "Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30771-0
    DOI: 10.1038/s41467-022-30771-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30771-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30771-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Huihui Wu & Bosheng Li & Hiro-oki Iwakawa & Yajie Pan & Xianli Tang & Qianyan Ling-hu & Yuelin Liu & Shixin Sheng & Li Feng & Hong Zhang & Xinyan Zhang & Zhonghua Tang & Xinli Xia & Jixian Zhai & Hong, 2020. "Plant 22-nt siRNAs mediate translational repression and stress adaptation," Nature, Nature, vol. 581(7806), pages 89-93, May.
    2. Yongqiang Liu & Hongru Wang & Zhimin Jiang & Wei Wang & Ruineng Xu & Qihui Wang & Zhihua Zhang & Aifu Li & Yan Liang & Shujun Ou & Xiujie Liu & Shouyun Cao & Hongning Tong & Yonghong Wang & Feng Zhou , 2021. "Genomic basis of geographical adaptation to soil nitrogen in rice," Nature, Nature, vol. 590(7847), pages 600-605, February.
    3. David Baulcombe, 2004. "RNA silencing in plants," Nature, Nature, vol. 431(7006), pages 356-363, September.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Xin Tong & Jia-Jia Zhao & Ya-Lan Feng & Jing-Ze Zou & Jian Ye & Junfeng Liu & Chenggui Han & Dawei Li & Xian-Bing Wang, 2023. "A selective autophagy receptor VISP1 induces symptom recovery by targeting viral silencing suppressors," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Salman Naveed & Sachin Rustgi, 2023. "Functional Characterization of Candidate Genes, Gohir.D05G103700 and Gohir.D12G153600 , Identified through Expression QTL Analysis Using Virus-Induced Gene Silencing in Upland Cotton ( Gossypium hirsu," Agriculture, MDPI, vol. 13(5), pages 1-12, May.
    2. Yajun Gou & Yueqin Heng & Wenyan Ding & Canhong Xu & Qiushuang Tan & Yajing Li & Yudong Fang & Xiaoqing Li & Degui Zhou & Xinyu Zhu & Mingyue Zhang & Rongjian Ye & Haiyang Wang & Rongxin Shen, 2024. "Natural variation in OsMYB8 confers diurnal floret opening time divergence between indica and japonica subspecies," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Bangjun Zhou & Huihui Yu & Yong Xue & Mu Li & Chi Zhang & Bin Yu, 2024. "The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Malathy Palayam & Linyi Yan & Ugrappa Nagalakshmi & Amelia K. Gilio & David Cornu & François-Didier Boyer & Savithramma P. Dinesh-Kumar & Nitzan Shabek, 2024. "Structural insights into strigolactone catabolism by carboxylesterases reveal a conserved conformational regulation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    5. Daai Zhang & Chengcheng Zhong & Neil A. Smith & Robert de Feyter & Ian K. Greaves & Steve M. Swain & Ren Zhang & Ming-Bo Wang, 2022. "Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    6. Adeeb Rahman & Neeti Sanan-Mishra, 2024. "When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants," Agriculture, MDPI, vol. 14(2), pages 1-26, February.
    7. Han Yang & Yafei Li & Yiwei Cao & Wenqing Shi & En Xie & Na Mu & Guijie Du & Yi Shen & Ding Tang & Zhukuan Cheng, 2022. "Nitrogen nutrition contributes to plant fertility by affecting meiosis initiation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    8. Geng Tian & Shubin Wang & Jianhui Wu & Yanxia Wang & Xiutang Wang & Shuwei Liu & Dejun Han & Guangmin Xia & Mengcheng Wang, 2023. "Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30771-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.