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Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana

Author

Listed:
  • Si Liu

    (University of California)

  • Meijuan Chen

    (University of California)

  • Ruidong Li

    (University of California)

  • Wan-Xiang Li

    (University of California)

  • Amit Gal-On

    (Volcani Center)

  • Zhenyu Jia

    (University of California)

  • Shou-Wei Ding

    (University of California)

Abstract

Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant virus resistance are enriched in genes of RNA interference (RNAi) pathway known to confer essential antiviral defense in plants. Here, we report two genome-wide association study screens to interrogate natural variation among wild-collected Arabidopsis thaliana accessions in quantitative resistance to the endemic cucumber mosaic virus (CMV). We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0. One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs). Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance. Our findings identify positive and negative regulators of the antiviral RNAi defense that may play important roles in virus-host coevolution.

Suggested Citation

  • Si Liu & Meijuan Chen & Ruidong Li & Wan-Xiang Li & Amit Gal-On & Zhenyu Jia & Shou-Wei Ding, 2022. "Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30771-0
    DOI: 10.1038/s41467-022-30771-0
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    References listed on IDEAS

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    1. Huihui Wu & Bosheng Li & Hiro-oki Iwakawa & Yajie Pan & Xianli Tang & Qianyan Ling-hu & Yuelin Liu & Shixin Sheng & Li Feng & Hong Zhang & Xinyan Zhang & Zhonghua Tang & Xinli Xia & Jixian Zhai & Hong, 2020. "Plant 22-nt siRNAs mediate translational repression and stress adaptation," Nature, Nature, vol. 581(7806), pages 89-93, May.
    2. Yongqiang Liu & Hongru Wang & Zhimin Jiang & Wei Wang & Ruineng Xu & Qihui Wang & Zhihua Zhang & Aifu Li & Yan Liang & Shujun Ou & Xiujie Liu & Shouyun Cao & Hongning Tong & Yonghong Wang & Feng Zhou , 2021. "Genomic basis of geographical adaptation to soil nitrogen in rice," Nature, Nature, vol. 590(7847), pages 600-605, February.
    3. David Baulcombe, 2004. "RNA silencing in plants," Nature, Nature, vol. 431(7006), pages 356-363, September.
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    2. Xin Tong & Jia-Jia Zhao & Ya-Lan Feng & Jing-Ze Zou & Jian Ye & Junfeng Liu & Chenggui Han & Dawei Li & Xian-Bing Wang, 2023. "A selective autophagy receptor VISP1 induces symptom recovery by targeting viral silencing suppressors," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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