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Signal-processing and adaptive prototissue formation in metabolic DNA protocells

Author

Listed:
  • Avik Samanta

    (A3BMS Lab, University of Mainz, Department of Chemistry)

  • Maximilian Hörner

    (University of Freiburg)

  • Wei Liu

    (A3BMS Lab, University of Mainz, Department of Chemistry)

  • Wilfried Weber

    (University of Freiburg)

  • Andreas Walther

    (A3BMS Lab, University of Mainz, Department of Chemistry
    University of Freiburg)

Abstract

The fundamental life-defining processes in living cells, such as replication, division, adaptation, and tissue formation, occur via intertwined metabolic reaction networks that process signals for downstream effects with high precision in a confined, crowded environment. Hence, it is crucial to understand and reenact some of these functions in wholly synthetic cell-like entities (protocells) to envision designing soft materials with life-like traits. Herein, we report on all-DNA protocells composed of a liquid DNA interior and a hydrogel-like shell, harboring a catalytically active DNAzyme, that converts DNA signals into functional metabolites that lead to downstream adaptation processes via site-selective strand displacement reactions. The downstream processes include intra-protocellular phenotype-like changes, prototissue formation via multivalent interactions, and chemical messenger communication between active sender and dormant receiver cell populations for sorted heteroprototissue formation. The approach integrates several tools of DNA-nanoscience in a synchronized way to mimic life-like behavior in artificial systems for future interactive materials.

Suggested Citation

  • Avik Samanta & Maximilian Hörner & Wei Liu & Wilfried Weber & Andreas Walther, 2022. "Signal-processing and adaptive prototissue formation in metabolic DNA protocells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31632-6
    DOI: 10.1038/s41467-022-31632-6
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    References listed on IDEAS

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    1. Daniela Sorrentino & Simona Ranallo & Francesco Ricci & Elisa Franco, 2024. "Developmental assembly of multi-component polymer systems through interconnected synthetic gene networks in vitro," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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