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Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus

Author

Listed:
  • Matthew Herdman

    (University of Oxford)

  • Buse Isbilir

    (MRC Laboratory of Molecular Biology)

  • Andriko Kügelgen

    (University of Oxford
    MRC Laboratory of Molecular Biology)

  • Ulrike Schulze

    (MRC Laboratory of Molecular Biology)

  • Alan Wainman

    (University of Oxford)

  • Tanmay A. M. Bharat

    (MRC Laboratory of Molecular Biology)

Abstract

Surface layers (S-layers) are proteinaceous, two-dimensional paracrystalline arrays that constitute a major component of the cell envelope in many prokaryotic species. In this study, we investigated S-layer biogenesis in the bacterial model organism Caulobacter crescentus. Fluorescence microscopy revealed localised incorporation of new S-layer at the poles and mid-cell, consistent with regions of cell growth in the cell cycle. Light microscopy and electron cryotomography investigations of drug-treated bacteria revealed that localised S-layer insertion is retained when cell division is inhibited, but is disrupted upon dysregulation of MreB or lipopolysaccharide. We further uncovered that S-layer biogenesis follows new peptidoglycan synthesis and localises to regions of high cell wall turnover. Finally, correlated cryo-light microscopy and electron cryotomographic analysis of regions of S-layer insertion showed the presence of discontinuities in the hexagonal S-layer lattice, contrasting with other S-layers completed by defined symmetric defects. Our findings present insights into how C. crescentus cells form an ordered S-layer on their surface in coordination with the biogenesis of other cell envelope components.

Suggested Citation

  • Matthew Herdman & Buse Isbilir & Andriko Kügelgen & Ulrike Schulze & Alan Wainman & Tanmay A. M. Bharat, 2024. "Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47529-5
    DOI: 10.1038/s41467-024-47529-5
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