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A de novo matrix for macroscopic living materials from bacteria

Author

Listed:
  • Sara Molinari

    (Rice University)

  • Robert F. Tesoriero

    (Rice University
    Rice University)

  • Dong Li

    (Lawrence Berkeley National Laboratory)

  • Swetha Sridhar

    (Rice University
    Rice University)

  • Rong Cai

    (Rice University)

  • Jayashree Soman

    (Rice University)

  • Kathleen R. Ryan

    (University of California, Berkeley)

  • Paul D. Ashby

    (Lawrence Berkeley National Laboratory)

  • Caroline M. Ajo-Franklin

    (Rice University)

Abstract

Engineered living materials (ELMs) embed living cells in a biopolymer matrix to create materials with tailored functions. While bottom-up assembly of macroscopic ELMs with a de novo matrix would offer the greatest control over material properties, we lack the ability to genetically encode a protein matrix that leads to collective self-organization. Here we report growth of ELMs from Caulobacter crescentus cells that display and secrete a self-interacting protein. This protein formed a de novo matrix and assembled cells into centimeter-scale ELMs. Discovery of design and assembly principles allowed us to tune the composition, mechanical properties, and catalytic function of these ELMs. This work provides genetic tools, design and assembly rules, and a platform for growing ELMs with control over both matrix and cellular structure and function.

Suggested Citation

  • Sara Molinari & Robert F. Tesoriero & Dong Li & Swetha Sridhar & Rong Cai & Jayashree Soman & Kathleen R. Ryan & Paul D. Ashby & Caroline M. Ajo-Franklin, 2022. "A de novo matrix for macroscopic living materials from bacteria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33191-2
    DOI: 10.1038/s41467-022-33191-2
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    References listed on IDEAS

    as
    1. Alexandre Persat & Howard A. Stone & Zemer Gitai, 2014. "The curved shape of Caulobacter crescentus enhances surface colonization in flow," Nature Communications, Nature, vol. 5(1), pages 1-9, September.
    2. Sun-Young Kang & Anaya Pokhrel & Sara Bratsch & Joey J. Benson & Seung-Oh Seo & Maureen B. Quin & Alptekin Aksan & Claudia Schmidt-Dannert, 2021. "Engineering Bacillus subtilis for the formation of a durable living biocomposite material," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    3. Anna M. Duraj-Thatte & Avinash Manjula-Basavanna & Jarod Rutledge & Jing Xia & Shabir Hassan & Arjirios Sourlis & Andrés G. Rubio & Ami Lesha & Michael Zenkl & Anton Kan & David A. Weitz & Yu Shrike Z, 2021. "Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    4. Yu Wang & Yufeng Wang & Xiaolong Zheng & Étienne Ducrot & Jeremy S. Yodh & Marcus Weck & David J. Pine, 2015. "Crystallization of DNA-coated colloids," Nature Communications, Nature, vol. 6(1), pages 1-8, November.
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    Cited by:

    1. Matthew Herdman & Buse Isbilir & Andriko Kügelgen & Ulrike Schulze & Alan Wainman & Tanmay A. M. Bharat, 2024. "Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Avinash Manjula-Basavanna & Anna M. Duraj-Thatte & Neel S. Joshi, 2024. "Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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