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Site-selected in situ polymerization for living cell surface engineering

Author

Listed:
  • Yihong Zhong

    (Nanjing University)

  • Lijia Xu

    (Nanjing University)

  • Chen Yang

    (Nanjing University)

  • Le Xu

    (Nanjing Tech University)

  • Guyu Wang

    (Nanjing University)

  • Yuna Guo

    (Nanjing University)

  • Songtao Cheng

    (Nanjing University)

  • Xiao Tian

    (Nanjing University)

  • Changjiang Wang

    (Nanjing University)

  • Ran Xie

    (Nanjing University
    Nanjing University)

  • Xiaojian Wang

    (Nanjing Tech University)

  • Lin Ding

    (Nanjing University
    Nanjing University)

  • Huangxian Ju

    (Nanjing University)

Abstract

The construction of polymer-based mimicry on cell surface to manipulate cell behaviors and functions offers promising prospects in the field of biotechnology and cell therapy. However, precise control of polymer grafting sites is essential to successful implementation of biomimicry and functional modulation, which has been overlooked by most current research. Herein, we report a biological site-selected, in situ controlled radical polymerization platform for living cell surface engineering. The method utilizes metabolic labeling techniques to confine the growth sites of polymers and designs a Fenton-RAFT polymerization technique with cytocompatibility. Polymers grown at different sites (glycans, proteins, lipids) have different membrane retention time and exhibit differential effects on the recognition behaviors of cellular glycans. Of particular importance is the achievement of in situ copolymerization of glycomonomers on the outermost natural glycan sites of cell membrane, building a biomimetic glycocalyx with distinct recognition properties.

Suggested Citation

  • Yihong Zhong & Lijia Xu & Chen Yang & Le Xu & Guyu Wang & Yuna Guo & Songtao Cheng & Xiao Tian & Changjiang Wang & Ran Xie & Xiaojian Wang & Lin Ding & Huangxian Ju, 2023. "Site-selected in situ polymerization for living cell surface engineering," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43161-x
    DOI: 10.1038/s41467-023-43161-x
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    References listed on IDEAS

    as
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