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Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly

Author

Listed:
  • Colin J. Comerci

    (Stanford University
    Stanford University)

  • Jonathan Herrmann

    (Stanford University
    SLAC National Accelerator Laboratory)

  • Joshua Yoon

    (Stanford University
    Stanford University)

  • Fatemeh Jabbarpour

    (Stanford University
    SLAC National Accelerator Laboratory)

  • Xiaofeng Zhou

    (Stanford University)

  • John F. Nomellini

    (University of British Columbia)

  • John Smit

    (University of British Columbia)

  • Lucy Shapiro

    (Stanford University)

  • Soichi Wakatsuki

    (Stanford University
    SLAC National Accelerator Laboratory)

  • W. E. Moerner

    (Stanford University
    Stanford University
    Stanford University)

Abstract

Many bacteria and most archaea possess a crystalline protein surface layer (S-layer), which surrounds their growing and topologically complicated outer surface. Constructing a macromolecular structure of this scale generally requires localized enzymatic machinery, but a regulatory framework for S-layer assembly has not been identified. By labeling, superresolution imaging, and tracking the S-layer protein (SLP) from C. crescentus, we show that 2D protein self-assembly is sufficient to build and maintain the S-layer in living cells by efficient protein crystal nucleation and growth. We propose a model supported by single-molecule tracking whereby randomly secreted SLP monomers diffuse on the lipopolysaccharide (LPS) outer membrane until incorporated at the edges of growing 2D S-layer crystals. Surface topology creates crystal defects and boundaries, thereby guiding S-layer assembly. Unsupervised assembly poses challenges for therapeutics targeting S-layers. However, protein crystallization as an evolutionary driver rationalizes S-layer diversity and raises the potential for biologically inspired self-assembling macromolecular nanomaterials.

Suggested Citation

  • Colin J. Comerci & Jonathan Herrmann & Joshua Yoon & Fatemeh Jabbarpour & Xiaofeng Zhou & John F. Nomellini & John Smit & Lucy Shapiro & Soichi Wakatsuki & W. E. Moerner, 2019. "Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10650-x
    DOI: 10.1038/s41467-019-10650-x
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    Cited by:

    1. Matthew Herdman & Buse Isbilir & Andriko Kügelgen & Ulrike Schulze & Alan Wainman & Tanmay A. M. Bharat, 2024. "Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Christoph Buhlheller & Theo Sagmeister & Christoph Grininger & Nina Gubensäk & Uwe B. Sleytr & Isabel Usón & Tea Pavkov-Keller, 2024. "SymProFold: Structural prediction of symmetrical biological assemblies," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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