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Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems

Author

Listed:
  • Sally Wang

    (University of Maryland
    University of Maryland
    University of Maryland)

  • Chen-Yu Chen

    (University of Maryland
    University of Maryland
    University of Maryland)

  • John R. Rzasa

    (University of Maryland)

  • Chen-Yu Tsao

    (University of Maryland
    University of Maryland)

  • Jinyang Li

    (University of Maryland
    University of Maryland
    California Institute of Technology)

  • Eric VanArsdale

    (University of Maryland
    University of Maryland
    University of Maryland
    United States Naval Research Laboratory)

  • Eunkyoung Kim

    (University of Maryland
    University of Maryland)

  • Fauziah Rahma Zakaria

    (University of Maryland
    University of Maryland
    University of Maryland)

  • Gregory F. Payne

    (University of Maryland
    University of Maryland)

  • William E. Bentley

    (University of Maryland
    University of Maryland
    University of Maryland)

Abstract

Microelectronic devices can directly communicate with biology, as electronic information can be transmitted via redox reactions within biological systems. By engineering biology’s native redox networks, we enable electronic interrogation and control of biological systems at several hierarchical levels: proteins, cells, and cell consortia. First, electro-biofabrication facilitates on-device biological component assembly. Then, electrode-actuated redox data transmission and redox-linked synthetic biology allows programming of enzyme activity and closed-loop electrogenetic control of cellular function. Specifically, horseradish peroxidase is assembled onto interdigitated electrodes where electrode-generated hydrogen peroxide controls its activity. E. coli’s stress response regulon, oxyRS, is rewired to enable algorithm-based feedback control of gene expression, including an eCRISPR module that switches cell-cell quorum sensing communication from one autoinducer to another—creating an electronically controlled ‘bilingual’ cell. Then, these disparate redox-guided devices are wirelessly connected, enabling real-time communication and user-based control. We suggest these methodologies will help us to better understand and develop sophisticated control for biology.

Suggested Citation

  • Sally Wang & Chen-Yu Chen & John R. Rzasa & Chen-Yu Tsao & Jinyang Li & Eric VanArsdale & Eunkyoung Kim & Fauziah Rahma Zakaria & Gregory F. Payne & William E. Bentley, 2023. "Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-44223-w
    DOI: 10.1038/s41467-023-44223-w
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    References listed on IDEAS

    as
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    3. Narendranath Bhokisham & Eric VanArsdale & Kristina T. Stephens & Pricila Hauk & Gregory F. Payne & William E. Bentley, 2020. "A redox-based electrogenetic CRISPR system to connect with and control biological information networks," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
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