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A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing

Author

Listed:
  • Jinjian Huang

    (Nanjing University)

  • Rong Yang

    (Nanjing Tech University
    Fudan University)

  • Jiao Jiao

    (The First Affiliated Hospital of Nanjing Medical University)

  • Ze Li

    (Nanjing University)

  • Penghui Wang

    (Nanjing Tech University)

  • Ye Liu

    (Southeast University)

  • Sicheng Li

    (Nanjing University)

  • Canwen Chen

    (Nanjing University)

  • Zongan Li

    (Nanjing Normal University)

  • Guiwen Qu

    (Southeast University)

  • Kang Chen

    (Nanjing University)

  • Xiuwen Wu

    (Nanjing University)

  • Bo Chi

    (Nanjing Tech University)

  • Jianan Ren

    (Nanjing University)

Abstract

High glucose-induced vascular endothelial injury is a major pathological factor involved in non-healing diabetic wounds. To interrupt this pathological process, we design an all-peptide printable hydrogel platform based on highly efficient and precise one-step click chemistry of thiolated γ-polyglutamic acid, glycidyl methacrylate-conjugated γ-polyglutamic acid, and thiolated arginine-glycine-aspartate sequences. Vascular endothelial growth factor 165-overexpressed human umbilical vein endothelial cells are printed using this platform, hence fabricating a living material with high cell viability and precise cell spatial distribution control. This cell-laden hydrogel platform accelerates the diabetic wound healing of rats based on the unabated vascular endothelial growth factor 165 release, which promotes angiogenesis and alleviates damages on vascular endothelial mitochondria, thereby reducing tissue hypoxia, downregulating inflammation, and facilitating extracellular matrix remodeling. Together, this study offers a promising strategy for fabricating tissue-friendly, high-efficient, and accurate 3D printed all-peptide hydrogel platform for cell delivery and self-renewable growth factor therapy.

Suggested Citation

  • Jinjian Huang & Rong Yang & Jiao Jiao & Ze Li & Penghui Wang & Ye Liu & Sicheng Li & Canwen Chen & Zongan Li & Guiwen Qu & Kang Chen & Xiuwen Wu & Bo Chi & Jianan Ren, 2023. "A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43364-2
    DOI: 10.1038/s41467-023-43364-2
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    1. Jianxiang Cheng & Rong Wang & Zechu Sun & Qingjiang Liu & Xiangnan He & Honggeng Li & Haitao Ye & Xingxin Yang & Xinfeng Wei & Zhenqing Li & Bingcong Jian & Weiwei Deng & Qi Ge, 2022. "Centrifugal multimaterial 3D printing of multifunctional heterogeneous objects," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Michael T. Lin & M. Flint Beal, 2006. "Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases," Nature, Nature, vol. 443(7113), pages 787-795, October.
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    Cited by:

    1. Ming Li & Chenxi Wang & Qiang Yu & Haoyi Chen & Yingying Ma & Li Wei & Mei X. Wu & Min Yao & Min Lu, 2024. "A wearable and stretchable dual-wavelength LED device for home care of chronic infected wounds," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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