IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-29908-y.html
   My bibliography  Save this article

Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata

Author

Listed:
  • Yu Wang

    (Northeast Forestry University
    Northeast Forestry University)

  • He Zhang

    (Northeast Forestry University
    Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education)

  • Hyok Chol Ri

    (Northeast Forestry University
    University of Science)

  • Zeyu An

    (Northeast Forestry University)

  • Xin Wang

    (Northeast Forestry University)

  • Jia-Nan Zhou

    (Northeast Forestry University)

  • Dongran Zheng

    (Northeast Forestry University)

  • Hao Wu

    (Northeast Forestry University
    Northeast Forestry University)

  • Pengchao Wang

    (Northeast Forestry University)

  • Jianfei Yang

    (Northeast Forestry University)

  • Ding-Kun Liu

    (Fujian Agriculture and Forestry University)

  • Diyang Zhang

    (Fujian Agriculture and Forestry University)

  • Wen-Chieh Tsai

    (National Cheng Kung University
    National Cheng Kung University)

  • Zheyong Xue

    (Northeast Forestry University
    Northeast Forestry University)

  • Zhichao Xu

    (Northeast Forestry University
    Northeast Forestry University)

  • Peng Zhang

    (Northeast Forestry University)

  • Zhong-Jian Liu

    (Fujian Agriculture and Forestry University)

  • Hailong Shen

    (Northeast Forestry University
    Northeast Forestry University)

  • Yuhua Li

    (Northeast Forestry University
    Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education)

Abstract

Araliaceae species produce various classes of triterpene and triterpenoid saponins, such as the oleanane-type triterpenoids in Aralia species and dammarane-type saponins in Panax, valued for their medicinal properties. The lack of genome sequences of Panax relatives has hindered mechanistic insight into the divergence of triterpene saponins in Araliaceae. Here, we report a chromosome-level genome of Aralia elata with a total length of 1.05 Gb. The loss of 12 exons in the dammarenediol synthase (DDS)-encoding gene in A. elata after divergence from Panax might have caused the lack of dammarane-type saponin production, and a complementation assay shows that overexpression of the PgDDS gene from Panax ginseng in callus of A. elata recovers the accumulation of dammarane-type saponins. Tandem duplication events of triterpene biosynthetic genes are common in the A. elata genome, especially for AeCYP72As, AeCSLMs, and AeUGT73s, which function as tailoring enzymes of oleanane-type saponins and aralosides. More than 13 aralosides are de novo synthesized in Saccharomyces cerevisiae by overexpression of these genes in combination. This study sheds light on the diversity of saponins biosynthetic pathway in Araliaceae and will facilitate heterologous bioproduction of aralosides.

Suggested Citation

  • Yu Wang & He Zhang & Hyok Chol Ri & Zeyu An & Xin Wang & Jia-Nan Zhou & Dongran Zheng & Hao Wu & Pengchao Wang & Jianfei Yang & Ding-Kun Liu & Diyang Zhang & Wen-Chieh Tsai & Zheyong Xue & Zhichao Xu , 2022. "Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29908-y
    DOI: 10.1038/s41467-022-29908-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-29908-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-29908-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Yu Wang & He Zhang & Hyok Chol Ri & Zeyu An & Xin Wang & Jia-Nan Zhou & Dongran Zheng & Hao Wu & Pengchao Wang & Jianfei Yang & Ding-Kun Liu & Diyang Zhang & Wen-Chieh Tsai & Zheyong Xue & Zhichao Xu , 2022. "Author Correction: Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata," Nature Communications, Nature, vol. 13(1), pages 1-2, December.
    2. Soo Yeon Chung & Hikaru Seki & Yukiko Fujisawa & Yoshikazu Shimoda & Susumu Hiraga & Yuhta Nomura & Kazuki Saito & Masao Ishimoto & Toshiya Muranaka, 2020. "A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jiao Yang & Ying Wu & Pan Zhang & Jianxiang Ma & Ying Jun Yao & Yan Lin Ma & Lei Zhang & Yongzhi Yang & Changmin Zhao & Jihua Wu & Xiangwen Fang & Jianquan Liu, 2023. "Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Wei Sun & Qinggang Yin & Huihua Wan & Ranran Gao & Chao Xiong & Chong Xie & Xiangxiao Meng & Yaolei Mi & Xiaotong Wang & Caixia Wang & Weiqiang Chen & Ziyan Xie & Zheyong Xue & Hui Yao & Peng Sun & Xu, 2023. "Characterization of the horse chestnut genome reveals the evolution of aescin and aesculin biosynthesis," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wei Sun & Qinggang Yin & Huihua Wan & Ranran Gao & Chao Xiong & Chong Xie & Xiangxiao Meng & Yaolei Mi & Xiaotong Wang & Caixia Wang & Weiqiang Chen & Ziyan Xie & Zheyong Xue & Hui Yao & Peng Sun & Xu, 2023. "Characterization of the horse chestnut genome reveals the evolution of aescin and aesculin biosynthesis," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Jiao Yang & Ying Wu & Pan Zhang & Jianxiang Ma & Ying Jun Yao & Yan Lin Ma & Lei Zhang & Yongzhi Yang & Changmin Zhao & Jihua Wu & Xiangwen Fang & Jianquan Liu, 2023. "Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Xiaoyue Chen & Graham A. Hudson & Charlotte Mineo & Bashar Amer & Edward E. K. Baidoo & Samantha A. Crowe & Yuzhong Liu & Jay D. Keasling & Henrik V. Scheller, 2023. "Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29908-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.