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Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls

Author

Listed:
  • Yoshihisa Yoshimi

    (Tennis Court Road)

  • Li Yu

    (Tennis Court Road)

  • Rosalie Cresswell

    (University of Warwick)

  • Xinyu Guo

    (Tennis Court Road)

  • Alberto Echevarría-Poza

    (Tennis Court Road)

  • Jan J. Lyczakowski

    (Jagiellonian University)

  • Ray Dupree

    (University of Warwick)

  • Toshihisa Kotake

    (Saitama University)

  • Paul Dupree

    (Tennis Court Road)

Abstract

Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate interaction with cellulose fibrils is poorly understood. Glucomannan backbones acquire variable patterns of galactosyl substitutions, depending on plant developmental stage and species. Here, we show that fine-tuning of galactosyl modification on glucomannans is achieved by the differing acceptor recognition of mannan α-galactosyltransferases (MAGTs). Biochemical analysis and 13C solid-state nuclear magnetic resonance spectroscopy of Arabidopsis with cell wall glucomannan engineered by MAGTs reveal that the degree of galactosylation strongly affects the interaction with cellulose. The findings indicate that plants tailor galactosyl modification on glucomannans for constructing an appropriate cell wall architecture, paving the way to convert properties of lignocellulosic biomass for better use.

Suggested Citation

  • Yoshihisa Yoshimi & Li Yu & Rosalie Cresswell & Xinyu Guo & Alberto Echevarría-Poza & Jan J. Lyczakowski & Ray Dupree & Toshihisa Kotake & Paul Dupree, 2025. "Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-56626-y
    DOI: 10.1038/s41467-025-56626-y
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    References listed on IDEAS

    as
    1. Jennie Berglund & Deirdre Mikkelsen & Bernadine M. Flanagan & Sushil Dhital & Stefan Gaunitz & Gunnar Henriksson & Mikael E. Lindström & Gleb E. Yakubov & Michael J. Gidley & Francisco Vilaplana, 2020. "Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    2. Oliver M. Terrett & Jan J. Lyczakowski & Li Yu & Dinu Iuga & W. Trent Franks & Steven P. Brown & Ray Dupree & Paul Dupree, 2019. "Molecular architecture of softwood revealed by solid-state NMR," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    3. Thomas J. Simmons & Jenny C. Mortimer & Oigres D. Bernardinelli & Ann-Christin Pöppler & Steven P. Brown & Eduardo R. deAzevedo & Ray Dupree & Paul Dupree, 2016. "Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR," Nature Communications, Nature, vol. 7(1), pages 1-9, December.
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