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Mapping diversity in African trypanosomes using high resolution spatial proteomics

Author

Listed:
  • Nicola M. Moloney

    (University of Cambridge)

  • Konstantin Barylyuk

    (University of Cambridge)

  • Eelco Tromer

    (University of Groningen)

  • Oliver M. Crook

    (University of Cambridge
    University of Oxford)

  • Lisa M. Breckels

    (University of Cambridge)

  • Kathryn S. Lilley

    (University of Cambridge)

  • Ross F. Waller

    (University of Cambridge)

  • Paula MacGregor

    (University of Cambridge
    University of Bristol)

Abstract

African trypanosomes are dixenous eukaryotic parasites that impose a significant human and veterinary disease burden on sub-Saharan Africa. Diversity between species and life-cycle stages is concomitant with distinct host and tissue tropisms within this group. Here, the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, are mapped across two life-stages. The four resulting datasets provide evidence of expression of approximately 5500 proteins per cell-type. Over 2500 proteins per cell-type are classified to specific subcellular compartments, providing four comprehensive spatial proteomes. Comparative analysis reveals key routes of parasitic adaptation to different biological niches and provides insight into the molecular basis for diversity within and between these pathogen species.

Suggested Citation

  • Nicola M. Moloney & Konstantin Barylyuk & Eelco Tromer & Oliver M. Crook & Lisa M. Breckels & Kathryn S. Lilley & Ross F. Waller & Paula MacGregor, 2023. "Mapping diversity in African trypanosomes using high resolution spatial proteomics," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40125-z
    DOI: 10.1038/s41467-023-40125-z
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    References listed on IDEAS

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    1. Mathieu Cayla & Christos Spanos & Kirsty McWilliam & Eliza Waskett & Juri Rappsilber & Keith R. Matthews, 2024. "Differentiation granules, a dynamic regulator of T. brucei development," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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