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Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition

Author

Listed:
  • Arsheed H. Sheikh

    (University of Warwick
    King Abdullah University of Science and Technology)

  • Iosif Zacharia

    (University of Warwick)

  • Alonso J. Pardal

    (University of Warwick)

  • Ana Dominguez-Ferreras

    (University of Warwick)

  • Daniela J. Sueldo

    (University of Warwick
    Norwegian University of Science and Technology)

  • Jung-Gun Kim

    (Stanford University)

  • Alexi Balmuth

    (J.R. Simplot Company
    Norwich Research Park)

  • Jose R. Gutierrez

    (Norwich Research Park)

  • Brendon F. Conlan

    (The Australian National University)

  • Najeeb Ullah

    (University of Warwick)

  • Olivia M. Nippe

    (University of Warwick)

  • Anil M. Girija

    (Tel-Aviv University)

  • Chih-Hang Wu

    (Norwich Research Park)

  • Guido Sessa

    (Tel-Aviv University)

  • Alexandra M. E. Jones

    (University of Warwick)

  • Murray R. Grant

    (University of Warwick)

  • Miriam L. Gifford

    (University of Warwick
    University of Warwick)

  • Mary Beth Mudgett

    (Stanford University)

  • John P. Rathjen

    (The Australian National University)

  • Vardis Ntoukakis

    (University of Warwick
    University of Warwick)

Abstract

In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of both the NLR complex and the protein kinase MAPKKKα. Moreover, we identified the helper NRC proteins (NLR-required for cell death) as integral components of the Prf /Pto NLR recognition complex. Notably our studies revealed that TFTs and NRCs interact with distinct modules of the NLR complex and, following effector recognition, dissociate facilitating downstream signalling. Thus, our data provide a mechanistic link between activation of immune receptors and initiation of downstream signalling cascades.

Suggested Citation

  • Arsheed H. Sheikh & Iosif Zacharia & Alonso J. Pardal & Ana Dominguez-Ferreras & Daniela J. Sueldo & Jung-Gun Kim & Alexi Balmuth & Jose R. Gutierrez & Brendon F. Conlan & Najeeb Ullah & Olivia M. Nip, 2023. "Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38103-6
    DOI: 10.1038/s41467-023-38103-6
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    References listed on IDEAS

    as
    1. Simon B. Saucet & Yan Ma & Panagiotis F. Sarris & Oliver J. Furzer & Kee Hoon Sohn & Jonathan D.G. Jones, 2015. "Two linked pairs of Arabidopsis TNL resistance genes independently confer recognition of bacterial effector AvrRps4," Nature Communications, Nature, vol. 6(1), pages 1-12, May.
    2. Smyth Gordon K, 2004. "Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments," Statistical Applications in Genetics and Molecular Biology, De Gruyter, vol. 3(1), pages 1-28, February.
    3. Jonathan D. G. Jones & Jeffery L. Dangl, 2006. "The plant immune system," Nature, Nature, vol. 444(7117), pages 323-329, November.
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    Cited by:

    1. Fabien Lonjon & Yan Lai & Nasrin Askari & Niharikaa Aiyar & Cedoljub Bundalovic-Torma & Bradley Laflamme & Pauline W. Wang & Darrell Desveaux & David S. Guttman, 2024. "The effector-triggered immunity landscape of tomato against Pseudomonas syringae," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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