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CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems

Author

Listed:
  • Marcus Ziemann

    (University of Freiburg)

  • Viktoria Reimann

    (University of Freiburg)

  • Yajing Liang

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory)

  • Yue Shi

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory)

  • Honglei Ma

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Yuman Xie

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Hui Li

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Tao Zhu

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Xuefeng Lu

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Wolfgang R. Hess

    (University of Freiburg)

Abstract

Certain CRISPR-Cas elements integrate into Tn7-like transposons, forming CRISPR-associated transposon (CAST) systems. How the activity of these systems is controlled in situ has remained largely unknown. Here we characterize the MerR-type transcriptional regulator Alr3614 that is encoded by one of the CAST (AnCAST) system genes in the genome of cyanobacterium Anabaena sp. PCC 7120. We identify a number of Alr3614 homologs across cyanobacteria and suggest naming these regulators CvkR for Cas V-K repressors. Alr3614/CvkR is translated from leaderless mRNA and represses the AnCAST core modules cas12k and tnsB directly, and indirectly the abundance of the tracr-CRISPR RNA. We identify a widely conserved CvkR binding motif 5’-AnnACATnATGTnnT-3’. Crystal structure of CvkR at 1.6 Å resolution reveals that it comprises distinct dimerization and potential effector-binding domains and that it assembles into a homodimer, representing a discrete structural subfamily of MerR regulators. CvkR repressors are at the core of a widely conserved regulatory mechanism that controls type V-K CAST systems.

Suggested Citation

  • Marcus Ziemann & Viktoria Reimann & Yajing Liang & Yue Shi & Honglei Ma & Yuman Xie & Hui Li & Tao Zhu & Xuefeng Lu & Wolfgang R. Hess, 2023. "CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36542-9
    DOI: 10.1038/s41467-023-36542-9
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    References listed on IDEAS

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