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CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems

Author

Listed:
  • Marcus Ziemann

    (University of Freiburg)

  • Viktoria Reimann

    (University of Freiburg)

  • Yajing Liang

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory)

  • Yue Shi

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory)

  • Honglei Ma

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Yuman Xie

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Hui Li

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Tao Zhu

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Xuefeng Lu

    (Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences
    Shandong Energy Institute
    Qingdao New Energy Shandong Laboratory
    University of Chinese Academy of Sciences)

  • Wolfgang R. Hess

    (University of Freiburg)

Abstract

Certain CRISPR-Cas elements integrate into Tn7-like transposons, forming CRISPR-associated transposon (CAST) systems. How the activity of these systems is controlled in situ has remained largely unknown. Here we characterize the MerR-type transcriptional regulator Alr3614 that is encoded by one of the CAST (AnCAST) system genes in the genome of cyanobacterium Anabaena sp. PCC 7120. We identify a number of Alr3614 homologs across cyanobacteria and suggest naming these regulators CvkR for Cas V-K repressors. Alr3614/CvkR is translated from leaderless mRNA and represses the AnCAST core modules cas12k and tnsB directly, and indirectly the abundance of the tracr-CRISPR RNA. We identify a widely conserved CvkR binding motif 5’-AnnACATnATGTnnT-3’. Crystal structure of CvkR at 1.6 Å resolution reveals that it comprises distinct dimerization and potential effector-binding domains and that it assembles into a homodimer, representing a discrete structural subfamily of MerR regulators. CvkR repressors are at the core of a widely conserved regulatory mechanism that controls type V-K CAST systems.

Suggested Citation

  • Marcus Ziemann & Viktoria Reimann & Yajing Liang & Yue Shi & Honglei Ma & Yuman Xie & Hui Li & Tao Zhu & Xuefeng Lu & Wolfgang R. Hess, 2023. "CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36542-9
    DOI: 10.1038/s41467-023-36542-9
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    References listed on IDEAS

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    1. Chengli Fang & Linyu Li & Yihan Zhao & Xiaoxian Wu & Steven J. Philips & Linlin You & Mingkang Zhong & Xiaojin Shi & Thomas V. O’Halloran & Qunyi Li & Yu Zhang, 2020. "The bacterial multidrug resistance regulator BmrR distorts promoter DNA to activate transcription," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    2. Ekaterina E. Zheleznova Heldwein & Richard G. Brennan, 2001. "Crystal structure of the transcription activator BmrR bound to DNA and a drug," Nature, Nature, vol. 409(6818), pages 378-382, January.
    3. Irma Querques & Michael Schmitz & Seraina Oberli & Christelle Chanez & Martin Jinek, 2021. "Target site selection and remodelling by type V CRISPR-transposon systems," Nature, Nature, vol. 599(7885), pages 497-502, November.
    4. Feng Wang & Qing He & Jia Yin & Sujuan Xu & Wei Hu & Lichuan Gu, 2018. "BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    5. Brady A. Travis & Jared V. Peck & Raul Salinas & Brandon Dopkins & Nicholas Lent & Viet D. Nguyen & Mario J. Borgnia & Richard G. Brennan & Maria A. Schumacher, 2022. "Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    6. Sanne E. Klompe & Phuc L. H. Vo & Tyler S. Halpin-Healy & Samuel H. Sternberg, 2019. "Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration," Nature, Nature, vol. 571(7764), pages 219-225, July.
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