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The bacterial multidrug resistance regulator BmrR distorts promoter DNA to activate transcription

Author

Listed:
  • Chengli Fang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Linyu Li

    (Fudan University)

  • Yihan Zhao

    (Henan University)

  • Xiaoxian Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Steven J. Philips

    (Northwestern University)

  • Linlin You

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Mingkang Zhong

    (Fudan University)

  • Xiaojin Shi

    (Fudan University)

  • Thomas V. O’Halloran

    (Northwestern University
    Northwestern University
    Northwestern University)

  • Qunyi Li

    (Fudan University)

  • Yu Zhang

    (Chinese Academy of Sciences)

Abstract

The MerR-family proteins represent a unique family of bacteria transcription factors (TFs), which activate transcription in a manner distinct from canonical ones. Here, we report a cryo-EM structure of a B. subtilis transcription activation complex comprising B. subtilis six-subunit (2αββ‘ωε) RNA Polymerase (RNAP) core enzyme, σA, a promoter DNA, and the ligand-bound B. subtilis BmrR, a prototype of MerR-family TFs. The structure reveals that RNAP and BmrR recognize the upstream promoter DNA from opposite faces and induce four significant kinks from the −35 element to the −10 element of the promoter DNA in a cooperative manner, which restores otherwise inactive promoter activity by shortening the length of promoter non-optimal −35/−10 spacer. Our structure supports a DNA-distortion and RNAP-non-contact paradigm of transcriptional activation by MerR TFs.

Suggested Citation

  • Chengli Fang & Linyu Li & Yihan Zhao & Xiaoxian Wu & Steven J. Philips & Linlin You & Mingkang Zhong & Xiaojin Shi & Thomas V. O’Halloran & Qunyi Li & Yu Zhang, 2020. "The bacterial multidrug resistance regulator BmrR distorts promoter DNA to activate transcription," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20134-y
    DOI: 10.1038/s41467-020-20134-y
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    Cited by:

    1. Marcus Ziemann & Viktoria Reimann & Yajing Liang & Yue Shi & Honglei Ma & Yuman Xie & Hui Li & Tao Zhu & Xuefeng Lu & Wolfgang R. Hess, 2023. "CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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