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BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin

Author

Listed:
  • Feng Wang

    (Shandong University)

  • Qing He

    (Shandong University)

  • Jia Yin

    (Shandong University)

  • Sujuan Xu

    (Shandong University)

  • Wei Hu

    (Shandong University)

  • Lichuan Gu

    (Shandong University)

Abstract

The virulence factor pyocyanin and the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP) play key roles in regulating biofilm formation and multi-drug efflux pump expression in Pseudomonas aeruginosa. However, the crosstalk between these two signaling pathways remains unclear. Here we show that BrlR (PA4878), previously identified as a c-di-GMP responsive transcriptional regulator, acts also as a receptor for pyocyanin. Crystal structures of free BrlR and c-di-GMP-bound BrlR reveal that the DNA-binding domain of BrlR contains two separate c-di-GMP binding sites, both of which are involved in promoting brlR expression. In addition, we identify a pyocyanin-binding site on the C-terminal multidrug-binding domain based on the structure of the BrlR-C domain in complex with a pyocyanin analog. Biochemical analysis indicates that pyocyanin enhances BrlR-DNA binding and brlR expression in a concentration-dependent manner.

Suggested Citation

  • Feng Wang & Qing He & Jia Yin & Sujuan Xu & Wei Hu & Lichuan Gu, 2018. "BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05004-y
    DOI: 10.1038/s41467-018-05004-y
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    Cited by:

    1. Marcus Ziemann & Viktoria Reimann & Yajing Liang & Yue Shi & Honglei Ma & Yuman Xie & Hui Li & Tao Zhu & Xuefeng Lu & Wolfgang R. Hess, 2023. "CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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