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Structures of the eukaryotic ribosome and its translational states in situ

Author

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  • Patrick C. Hoffmann

    (Max Planck Institute of Biophysics)

  • Jan Philipp Kreysing

    (Max Planck Institute of Biophysics
    IMPRS on Cellular Biophysics)

  • Iskander Khusainov

    (Max Planck Institute of Biophysics)

  • Maarten W. Tuijtel

    (Max Planck Institute of Biophysics)

  • Sonja Welsch

    (Max Planck Institute of Biophysics)

  • Martin Beck

    (Max Planck Institute of Biophysics)

Abstract

Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. Different translational states of ribosomes have been well characterized in vitro. However, to which extent the known translational states are representative of the native situation inside cells has thus far only been addressed in prokaryotes. Here, we apply cryo-electron tomography to cryo-FIB milled Dictyostelium discoideum cells combined with subtomogram averaging and classification. We obtain an in situ structure that is locally resolved up to 3 Angstrom, the distribution of eukaryotic ribosome translational states, and unique arrangement of rRNA expansion segments. Our work demonstrates the use of in situ structural biology techniques for identifying distinct ribosome states within the cellular environment.

Suggested Citation

  • Patrick C. Hoffmann & Jan Philipp Kreysing & Iskander Khusainov & Maarten W. Tuijtel & Sonja Welsch & Martin Beck, 2022. "Structures of the eukaryotic ribosome and its translational states in situ," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34997-w
    DOI: 10.1038/s41467-022-34997-w
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    Cited by:

    1. Xing Zhang & Yanan Xiao & Xin You & Shan Sun & Sen-Fang Sui, 2024. "In situ structural determination of cyanobacterial phycobilisome–PSII supercomplex by STAgSPA strategy," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Iskander Khusainov & Natalie Romanov & Camille Goemans & Beata Turoňová & Christian E. Zimmerli & Sonja Welsch & Julian D. Langer & Athanasios Typas & Martin Beck, 2024. "Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    3. Sergio Cruz-León & Tomáš Majtner & Patrick C. Hoffmann & Jan Philipp Kreysing & Sebastian Kehl & Maarten W. Tuijtel & Stefan L. Schaefer & Katharina Geißler & Martin Beck & Beata Turoňová & Gerhard Hu, 2024. "High-confidence 3D template matching for cryo-electron tomography," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    4. Jing Cheng & Tong Liu & Xin You & Fa Zhang & Sen-Fang Sui & Xiaohua Wan & Xinzheng Zhang, 2023. "Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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