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Structural landscape of the respiratory syncytial virus nucleocapsids

Author

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  • Lorène Gonnin

    (Univ Grenoble Alpes, CEA, CNRS, IBS
    Paris-Saclay University, INRAE)

  • Ambroise Desfosses

    (Univ Grenoble Alpes, CEA, CNRS, IBS)

  • Maria Bacia-Verloop

    (Univ Grenoble Alpes, CEA, CNRS, IBS)

  • Didier Chevret

    (Paris-Saclay University, INRAE)

  • Marie Galloux

    (Paris-Saclay University, INRAE)

  • Jean-François Éléouët

    (Paris-Saclay University, INRAE)

  • Irina Gutsche

    (Univ Grenoble Alpes, CEA, CNRS, IBS)

Abstract

Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation.

Suggested Citation

  • Lorène Gonnin & Ambroise Desfosses & Maria Bacia-Verloop & Didier Chevret & Marie Galloux & Jean-François Éléouët & Irina Gutsche, 2023. "Structural landscape of the respiratory syncytial virus nucleocapsids," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41439-8
    DOI: 10.1038/s41467-023-41439-8
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    References listed on IDEAS

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    Cited by:

    1. Bryan S. Sibert & Joseph Y. Kim & Jie E. Yang & Zunlong Ke & Christopher C. Stobart & Martin L. Moore & Elizabeth R. Wright, 2024. "Assembly of respiratory syncytial virus matrix protein lattice and its coordination with fusion glycoprotein trimers," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Jack D. Whitehead & Hortense Decool & Cédric Leyrat & Loic Carrique & Jenna Fix & Jean-François Eléouët & Marie Galloux & Max Renner, 2023. "Structure of the N-RNA/P interface indicates mode of L/P recruitment to the nucleocapsid of human metapneumovirus," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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