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Structures of the human and Drosophila 80S ribosome

Author

Listed:
  • Andreas M. Anger

    (Gene Center, Ludwig-Maximilians-Universität München)

  • Jean-Paul Armache

    (Gene Center, Ludwig-Maximilians-Universität München)

  • Otto Berninghausen

    (Gene Center, Ludwig-Maximilians-Universität München)

  • Michael Habeck

    (Max Planck Institute for Biological Cybernetics, Spemannstrasse 38, 72076 Tübingen, Germany
    Max Planck Institute for Developmental Biology, Spemannstrasse 38, 72076 Tübingen, Germany)

  • Marion Subklewe

    (Klinikum der Universität München and Clinical Cooperation Group Immunotherapy at the Helmholtz Institute Munich, Marchioninistrasse 15, 81377 Munich, Germany)

  • Daniel N. Wilson

    (Gene Center, Ludwig-Maximilians-Universität München)

  • Roland Beckmann

    (Gene Center, Ludwig-Maximilians-Universität München)

Abstract

Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

Suggested Citation

  • Andreas M. Anger & Jean-Paul Armache & Otto Berninghausen & Michael Habeck & Marion Subklewe & Daniel N. Wilson & Roland Beckmann, 2013. "Structures of the human and Drosophila 80S ribosome," Nature, Nature, vol. 497(7447), pages 80-85, May.
    • Handle: RePEc:nat:nature:v:497:y:2013:i:7447:d:10.1038_nature12104
    DOI: 10.1038/nature12104
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    Cited by:

    1. Patrick C. Hoffmann & Jan Philipp Kreysing & Iskander Khusainov & Maarten W. Tuijtel & Sonja Welsch & Martin Beck, 2022. "Structures of the eukaryotic ribosome and its translational states in situ," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Thu Giang Nguyen & Christina Ritter & Eva Kummer, 2023. "Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Ryan Damme & Kongpan Li & Minjie Zhang & Jianhui Bai & Wilson H. Lee & Joseph D. Yesselman & Zhipeng Lu & Willem A. Velema, 2022. "Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cells," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Claudia M. Fusco & Kristina Desch & Aline R. Dörrbaum & Mantian Wang & Anja Staab & Ivy C. W. Chan & Eleanor Vail & Veronica Villeri & Julian D. Langer & Erin M. Schuman, 2021. "Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    5. Silvia Martini & Khalil Davis & Rupert Faraway & Lisa Elze & Nicola Lockwood & Andrew Jones & Xiao Xie & Neil Q. McDonald & David J. Mann & Alan Armstrong & Jernej Ule & Peter J. Parker, 2021. "A genetically-encoded crosslinker screen identifies SERBP1 as a PKCε substrate influencing translation and cell division," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    6. Naomi R. Genuth & Zhen Shi & Koshi Kunimoto & Victoria Hung & Adele F. Xu & Craig H. Kerr & Gerald C. Tiu & Juan A. Oses-Prieto & Rachel E. A. Salomon-Shulman & Jeffrey D. Axelrod & Alma L. Burlingame, 2022. "A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    7. Yan Chen & Bin Tsai & Ningning Li & Ning Gao, 2022. "Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    8. Jong Woo Bae & Sangtae Kim & V. Narry Kim & Jong-Seo Kim, 2021. "Photoactivatable ribonucleosides mark base-specific RNA-binding sites," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    9. Ulrike Zinnall & Miha Milek & Igor Minia & Carlos H. Vieira-Vieira & Simon Müller & Guido Mastrobuoni & Orsalia-Georgia Hazapis & Simone Giudice & David Schwefel & Nadine Bley & Franka Voigt & Jeffrey, 2022. "HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    10. Patrick R. Smith & Sarah Loerch & Nikesh Kunder & Alexander D. Stanowick & Tzu-Fang Lou & Zachary T. Campbell, 2021. "Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    11. Kai Hao & Yawen Chen & Xiumin Yan & Xueliang Zhu, 2021. "Cilia locally synthesize proteins to sustain their ultrastructure and functions," Nature Communications, Nature, vol. 12(1), pages 1-16, December.

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