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Intracellular morphogenesis of diatom silica is guided by local variations in membrane curvature

Author

Listed:
  • Lior Aram

    (Weizmann Institute of Science)

  • Diede Haan

    (Weizmann Institute of Science)

  • Neta Varsano

    (Weizmann Institute of Science)

  • James B. Gilchrist

    (Diamond Light Source, Harwell Science and Innovation Campus)

  • Christoph Heintze

    (Technische Universität Dresden)

  • Ron Rotkopf

    (Weizmann Institute of Science)

  • Katya Rechav

    (Weizmann Institute of Science)

  • Nadav Elad

    (Weizmann Institute of Science)

  • Nils Kröger

    (Technische Universität Dresden
    Technische Universität Dresden
    Technische Universität Dresden)

  • Assaf Gal

    (Weizmann Institute of Science)

Abstract

Silica cell-wall formation in diatoms is a showcase for the ability of organisms to control inorganic mineralization. The process of silicification by these unicellular algae is tightly regulated within a membrane-bound organelle, the silica deposition vesicle (SDV). Two opposing scenarios were proposed to explain the tight regulation of this intracellular process: a template-mediated process that relies on preformed scaffolds, or a template-independent self-assembly process. The present work points to a third scenario, where the SDV membrane is a dynamic mold that shapes the forming silica. We use in-cell cryo-electron tomography to visualize the silicification process in situ, in its native-state, and with a nanometer-scale resolution. This reveals that the plasma membrane interacts with the SDV membrane via physical tethering at membrane contact sites, where the curvature of the tethered side of the SDV membrane mirrors the intricate silica topography. We propose that silica growth and morphogenesis result from the biophysical properties of the SDV and plasma membranes.

Suggested Citation

  • Lior Aram & Diede Haan & Neta Varsano & James B. Gilchrist & Christoph Heintze & Ron Rotkopf & Katya Rechav & Nadav Elad & Nils Kröger & Assaf Gal, 2024. "Intracellular morphogenesis of diatom silica is guided by local variations in membrane curvature," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52211-x
    DOI: 10.1038/s41467-024-52211-x
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    References listed on IDEAS

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    1. Diede Haan & Lior Aram & Hadas Peled-Zehavi & Yoseph Addadi & Oz Ben-Joseph & Ron Rotkopf & Nadav Elad & Katya Rechav & Assaf Gal, 2023. "Exocytosis of the silicified cell wall of diatoms involves extensive membrane disintegration," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Liang Xue & Swantje Lenz & Maria Zimmermann-Kogadeeva & Dimitry Tegunov & Patrick Cramer & Peer Bork & Juri Rappsilber & Julia Mahamid, 2022. "Publisher Correction: Visualizing translation dynamics at atomic detail inside a bacterial cell," Nature, Nature, vol. 611(7937), pages 13-13, November.
    3. Liang Xue & Swantje Lenz & Maria Zimmermann-Kogadeeva & Dimitry Tegunov & Patrick Cramer & Peer Bork & Juri Rappsilber & Julia Mahamid, 2022. "Visualizing translation dynamics at atomic detail inside a bacterial cell," Nature, Nature, vol. 610(7930), pages 205-211, October.
    4. Boaz Mayzel & Lior Aram & Neta Varsano & Sharon G. Wolf & Assaf Gal, 2021. "Structural evidence for extracellular silica formation by diatoms," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
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