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In vitro assembly, positioning and contraction of a division ring in minimal cells

Author

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  • Shunshi Kohyama

    (Max Planck Institute of Biochemistry)

  • Adrián Merino-Salomón

    (Max Planck Institute of Biochemistry)

  • Petra Schwille

    (Max Planck Institute of Biochemistry)

Abstract

Constructing a minimal machinery for autonomous self-division of synthetic cells is a major goal of bottom-up synthetic biology. One paradigm has been the E. coli divisome, with the MinCDE protein system guiding assembly and positioning of a presumably contractile ring based on FtsZ and its membrane adaptor FtsA. Here, we demonstrate the full in vitro reconstitution of this machinery consisting of five proteins within lipid vesicles, allowing to observe the following sequence of events in real time: 1) Assembly of an isotropic filamentous FtsZ network, 2) its condensation into a ring-like structure, along with pole-to-pole mode selection of Min oscillations resulting in equatorial positioning, and 3) onset of ring constriction, deforming the vesicles from spherical shape. Besides demonstrating these essential features, we highlight the importance of decisive experimental factors, such as macromolecular crowding. Our results provide an exceptional showcase of the emergence of cell division in a minimal system, and may represent a step towards developing a synthetic cell.

Suggested Citation

  • Shunshi Kohyama & Adrián Merino-Salomón & Petra Schwille, 2022. "In vitro assembly, positioning and contraction of a division ring in minimal cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33679-x
    DOI: 10.1038/s41467-022-33679-x
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    References listed on IDEAS

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    1. Elisa Godino & Jonás Noguera López & David Foschepoth & Céline Cleij & Anne Doerr & Clara Ferrer Castellà & Christophe Danelon, 2019. "De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    2. Thomas Litschel & Charlotte F. Kelley & Danielle Holz & Maral Adeli Koudehi & Sven K. Vogel & Laura Burbaum & Naoko Mizuno & Dimitrios Vavylonis & Petra Schwille, 2021. "Reconstitution of contractile actomyosin rings in vesicles," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    3. Beatrice Ramm & Philipp Glock & Jonas Mücksch & Philipp Blumhardt & Daniela A. García-Soriano & Michael Heymann & Petra Schwille, 2018. "The MinDE system is a generic spatial cue for membrane protein distribution in vitro," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    4. Diego A. Ramirez-Diaz & Adrián Merino-Salomón & Fabian Meyer & Michael Heymann & Germán Rivas & Marc Bramkamp & Petra Schwille, 2021. "FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
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    Cited by:

    1. Jingjing Zhao & Xiaojun Han, 2024. "Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Shunshi Kohyama & Béla P. Frohn & Leon Babl & Petra Schwille, 2024. "Machine learning-aided design and screening of an emergent protein function in synthetic cells," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Shubin Li & Yingming Zhao & Shuqi Wu & Xiangxiang Zhang & Boyu Yang & Liangfei Tian & Xiaojun Han, 2023. "Regulation of species metabolism in synthetic community systems by environmental pH oscillations," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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