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Kinetic trapping organizes actin filaments within liquid-like protein droplets

Author

Listed:
  • Aravind Chandrasekaran

    (University of California San Diego)

  • Kristin Graham

    (University of Texas at Austin)

  • Jeanne C. Stachowiak

    (University of Texas at Austin
    University of Texas at Austin)

  • Padmini Rangamani

    (University of California San Diego)

Abstract

Several actin-binding proteins (ABPs) phase separate to form condensates capable of curating the actin network shapes. Here, we use computational modeling to understand the principles of actin network organization within VASP condensate droplets. Our simulations reveal that the different actin shapes, namely shells, rings, and mixture states are highly dependent on the kinetics of VASP-actin interactions, suggesting that they arise from kinetic trapping. Specifically, we show that reducing the residence time of VASP on actin filaments reduces degree of bundling, thereby promoting assembly of shells rather than rings. We validate the model predictions experimentally using a VASP-mutant with decreased bundling capability. Finally, we investigate the ring opening within deformed droplets and found that the sphere-to-ellipsoid transition is favored under a wide range of filament lengths while the ellipsoid-to-rod transition is only permitted when filaments have a specific range of lengths. Our findings highlight key mechanisms of actin organization within phase-separated ABPs.

Suggested Citation

  • Aravind Chandrasekaran & Kristin Graham & Jeanne C. Stachowiak & Padmini Rangamani, 2024. "Kinetic trapping organizes actin filaments within liquid-like protein droplets," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46726-6
    DOI: 10.1038/s41467-024-46726-6
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    References listed on IDEAS

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    1. He Sun & Xinlu Zhu & Chuanxi Li & Zhiming Ma & Xiao Han & Yuanyuan Luo & Liang Yang & Jing Yu & Yansong Miao, 2021. "Xanthomonas effector XopR hijacks host actin cytoskeleton via complex coacervation," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
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    3. Thomas P. Loisel & Rajaa Boujemaa & Dominique Pantaloni & Marie-France Carlier, 1999. "Reconstitution of actin-based motility of Listeria and Shigella using pure proteins," Nature, Nature, vol. 401(6753), pages 613-616, October.
    4. Mélanie Chabaud & Mélina L. Heuzé & Marine Bretou & Pablo Vargas & Paolo Maiuri & Paola Solanes & Mathieu Maurin & Emmanuel Terriac & Maël Le Berre & Danielle Lankar & Tristan Piolot & Robert S. Adels, 2015. "Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells," Nature Communications, Nature, vol. 6(1), pages 1-16, November.
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