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De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns

Author

Listed:
  • Elisa Godino

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Jonás Noguera López

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • David Foschepoth

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Céline Cleij

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Anne Doerr

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Clara Ferrer Castellà

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Christophe Danelon

    (Kavli Institute of Nanoscience, Delft University of Technology)

Abstract

The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro.

Suggested Citation

  • Elisa Godino & Jonás Noguera López & David Foschepoth & Céline Cleij & Anne Doerr & Clara Ferrer Castellà & Christophe Danelon, 2019. "De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12932-w
    DOI: 10.1038/s41467-019-12932-w
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    Cited by:

    1. Shunshi Kohyama & Béla P. Frohn & Leon Babl & Petra Schwille, 2024. "Machine learning-aided design and screening of an emergent protein function in synthetic cells," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Sabrina Meindlhumer & Fridtjof Brauns & Jernej Rudi Finžgar & Jacob Kerssemakers & Cees Dekker & Erwin Frey, 2023. "Directing Min protein patterns with advective bulk flow," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    3. Shunshi Kohyama & Adrián Merino-Salomón & Petra Schwille, 2022. "In vitro assembly, positioning and contraction of a division ring in minimal cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Zhanar Abil & Ana María Restrepo Sierra & Andreea R. Stan & Amélie Châne & Alicia Prado & Miguel Vega & Yannick Rondelez & Christophe Danelon, 2024. "Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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