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The MinDE system is a generic spatial cue for membrane protein distribution in vitro

Author

Listed:
  • Beatrice Ramm

    (Max Planck Institute of Biochemistry)

  • Philipp Glock

    (Max Planck Institute of Biochemistry)

  • Jonas Mücksch

    (Max Planck Institute of Biochemistry)

  • Philipp Blumhardt

    (Max Planck Institute of Biochemistry)

  • Daniela A. García-Soriano

    (Max Planck Institute of Biochemistry)

  • Michael Heymann

    (Max Planck Institute of Biochemistry)

  • Petra Schwille

    (Max Planck Institute of Biochemistry)

Abstract

The E. coli MinCDE system has become a paradigmatic reaction–diffusion system in biology. The membrane-bound ATPase MinD and ATPase-activating protein MinE oscillate between the cell poles followed by MinC, thus positioning the main division protein FtsZ at midcell. Here we report that these energy-consuming MinDE oscillations may play a role beyond constraining MinC/FtsZ localization. Using an in vitro reconstitution assay, we show that MinDE self-organization can spatially regulate a variety of functionally completely unrelated membrane proteins into patterns and gradients. By concentration waves sweeping over the membrane, they induce a direct net transport of tightly membrane-attached molecules. That the MinDE system can spatiotemporally control a much larger set of proteins than previously known, may constitute a MinC-independent pathway to division site selection and chromosome segregation. Moreover, the here described phenomenon of active transport through a traveling diffusion barrier may point to a general mechanism of spatiotemporal regulation in cells.

Suggested Citation

  • Beatrice Ramm & Philipp Glock & Jonas Mücksch & Philipp Blumhardt & Daniela A. García-Soriano & Michael Heymann & Petra Schwille, 2018. "The MinDE system is a generic spatial cue for membrane protein distribution in vitro," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06310-1
    DOI: 10.1038/s41467-018-06310-1
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    Cited by:

    1. Shunshi Kohyama & Adrián Merino-Salomón & Petra Schwille, 2022. "In vitro assembly, positioning and contraction of a division ring in minimal cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Suin Shim & Bernardo Gouveia & Beatrice Ramm & Venecia A. Valdez & Sabine Petry & Howard A. Stone, 2024. "Motorless transport of microtubules along tubulin, RanGTP, and salt gradients," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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