IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-33156-5.html
   My bibliography  Save this article

Reconstitution of microtubule into GTP-responsive nanocapsules

Author

Listed:
  • Noriyuki Uchida

    (RIKEN Center for Emergent Matter Science
    Tokyo University of Agriculture and Technology)

  • Ai Kohata

    (The University of Tokyo)

  • Kou Okuro

    (The University of Hong Kong
    The University of Hong Kong)

  • Annalisa Cardellini

    (Politecnico di Torino)

  • Chiara Lionello

    (Politecnico di Torino)

  • Eric A. Zizzi

    (Politecnico di Torino)

  • Marco A. Deriu

    (Politecnico di Torino)

  • Giovanni M. Pavan

    (Politecnico di Torino
    University of Applied Sciences and Arts of Southern Switzerland, Polo Universitario Lugano, Campus Est)

  • Michio Tomishige

    (Aoyama Gakuin University)

  • Takaaki Hikima

    (RIKEN SPring-8 Center)

  • Takuzo Aida

    (RIKEN Center for Emergent Matter Science
    The University of Tokyo)

Abstract

Nanocapsules that collapse in response to guanosine triphosphate (GTP) have the potential as drug carriers for efficiently curing diseases caused by cancer and RNA viruses because GTP is present at high levels in such diseased cells and tissues. However, known GTP-responsive carriers also respond to adenosine triphosphate (ATP), which is abundant in normal cells as well. Here, we report the elaborate reconstitution of microtubule into a nanocapsule that selectively responds to GTP. When the tubulin monomer from microtubule is incubated at 37 °C with a mixture of GTP (17 mol%) and nonhydrolysable GTP* (83 mol%), a tubulin nanosheet forms. Upon addition of photoreactive molecular glue to the resulting dispersion, the nanosheet is transformed into a nanocapsule. Cell death results when a doxorubicin-containing nanocapsule, after photochemically crosslinked for properly stabilizing its shell, is taken up into cancer cells that overexpress GTP.

Suggested Citation

  • Noriyuki Uchida & Ai Kohata & Kou Okuro & Annalisa Cardellini & Chiara Lionello & Eric A. Zizzi & Marco A. Deriu & Giovanni M. Pavan & Michio Tomishige & Takaaki Hikima & Takuzo Aida, 2022. "Reconstitution of microtubule into GTP-responsive nanocapsules," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33156-5
    DOI: 10.1038/s41467-022-33156-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-33156-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-33156-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Hector Aldaz & Luke M. Rice & Tim Stearns & David A. Agard, 2005. "Insights into microtubule nucleation from the crystal structure of human γ-tubulin," Nature, Nature, vol. 435(7041), pages 523-527, May.
    2. Yuta Suzuki & Giovanni Cardone & David Restrepo & Pablo D. Zavattieri & Timothy S. Baker & F. Akif Tezcan, 2016. "Self-assembly of coherently dynamic, auxetic, two-dimensional protein crystals," Nature, Nature, vol. 533(7603), pages 369-373, May.
    3. Charles Sawyers, 2004. "Targeted cancer therapy," Nature, Nature, vol. 432(7015), pages 294-297, November.
    4. Andrew P. Carter & William M. Clemons & Ditlev E. Brodersen & Robert J. Morgan-Warren & Brian T. Wimberly & V. Ramakrishnan, 2000. "Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics," Nature, Nature, vol. 407(6802), pages 340-348, September.
    5. Reuben J. Shaw & Lewis C. Cantley, 2006. "Ras, PI(3)K and mTOR signalling controls tumour cell growth," Nature, Nature, vol. 441(7092), pages 424-430, May.
    6. Andrew C. Kruse & Aaron M. Ring & Aashish Manglik & Jianxin Hu & Kelly Hu & Katrin Eitel & Harald Hübner & Els Pardon & Celine Valant & Patrick M. Sexton & Arthur Christopoulos & Christian C. Felder &, 2013. "Activation and allosteric modulation of a muscarinic acetylcholine receptor," Nature, Nature, vol. 504(7478), pages 101-106, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yan Han & Ying Yuan & Sha Cao & Muyi Li & Yong Zang, 2020. "On the Use of Marker Strategy Design to Detect Predictive Marker Effect in Cancer Immunotherapy and Targeted Therapy," Statistics in Biosciences, Springer;International Chinese Statistical Association, vol. 12(2), pages 180-195, July.
    2. Yosuke Toyoda & Angqi Zhu & Fang Kong & Sisi Shan & Jiawei Zhao & Nan Wang & Xiaoou Sun & Linqi Zhang & Chuangye Yan & Brian K. Kobilka & Xiangyu Liu, 2023. "Structural basis of α1A-adrenergic receptor activation and recognition by an extracellular nanobody," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Yung Lee & Bongkyun Jang & Hyunggwi Song & Sumin Kim & Yong Won Kwon & Hyun Seok Kang & Min Seong Kim & Inkyu Park & Taek-Soo Kim & Junho Jang & Jae-Hyun Kim & Jang-Ung Park & Byeong-Soo Bae, 2024. "A seamless auxetic substrate with a negative Poisson’s ratio of −1," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    4. Thomas Fontaine & Andreas Busch & Toon Laeremans & Stéphane De Cesco & Yi-Lynn Liang & Veli-Pekka Jaakola & Zara Sands & Sarah Triest & Simonas Masiulis & Lies Dekeyzer & Noor Samyn & Nicolas Loeys & , 2024. "Structure elucidation of a human melanocortin-4 receptor specific orthosteric nanobody agonist," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    5. Yang Yang & Hye Jin Kang & Ruogu Gao & Jingjing Wang & Gye Won Han & Jeffrey F. DiBerto & Lijie Wu & Jiahui Tong & Lu Qu & Yiran Wu & Ryan Pileski & Xuemei Li & Xuejun Cai Zhang & Suwen Zhao & Terry K, 2023. "Structural insights into the human niacin receptor HCA2-Gi signalling complex," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    6. Rebecca S Mathew-Fenn & Rhiju Das & Joshua A Silverman & Peter A Walker & Pehr A B Harbury, 2008. "A Molecular Ruler for Measuring Quantitative Distance Distributions," PLOS ONE, Public Library of Science, vol. 3(10), pages 1-9, October.
    7. Yi Fu & Robert Kunz & Jianhua Wu & Cheng Dong, 2012. "Study of Local Hydrodynamic Environment in Cell-Substrate Adhesion Using Side-View μPIV Technology," PLOS ONE, Public Library of Science, vol. 7(2), pages 1-13, February.
    8. Kai Yuan & Zhaoxing Li & Wenbin Kuang & Xiao Wang & Minghui Ji & Weijiao Chen & Jiayu Ding & Jiaxing Li & Wenjian Min & Chengliang Sun & Xiuquan Ye & Meiling Lu & Liping Wang & Haixia Ge & Yuzhang Jia, 2022. "Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    9. Pengyi Yang & Xiaofeng Zheng & Vivek Jayaswal & Guang Hu & Jean Yee Hwa Yang & Raja Jothi, 2015. "Knowledge-Based Analysis for Detecting Key Signaling Events from Time-Series Phosphoproteomics Data," PLOS Computational Biology, Public Library of Science, vol. 11(8), pages 1-18, August.
    10. Sanahan Vijayakumar & Robert G. Alberstein & Zhiyin Zhang & Yi-Sheng Lu & Adriano Chan & Charlotte E. Wahl & James S. Ha & Deborah E. Hunka & Gerry R. Boss & Michael J. Sailor & F. Akif Tezcan, 2024. "Designed 2D protein crystals as dynamic molecular gatekeepers for a solid-state device," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    11. Joshua A. Lees & João M. Dias & Francis Rajamohan & Jean-Philippe Fortin & Rebecca O’Connor & Jimmy X. Kong & Emily A. G. Hughes & Ethan L. Fisher & Jamison B. Tuttle & Gabrielle Lovett & Bethany L. K, 2023. "An inverse agonist of orphan receptor GPR61 acts by a G protein-competitive allosteric mechanism," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    12. Wei-Xiang Qi & Yu-Jing Huang & Yang Yao & Zan Shen & Da-Liu Min, 2013. "Incidence and Risk of Treatment-Related Mortality with mTOR Inhibitors Everolimus and Temsirolimus in Cancer Patients: A Meta-Analysis," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-8, June.
    13. Guo-dong Cao & Xing-yu Xu & Jia-wei Zhang & Bo Chen & Mao-ming Xiong, 2016. "Phosphorylated Mammalian Target of Rapamycin p-mTOR Is a Favorable Prognostic Factor than mTOR in Gastric Cancer," PLOS ONE, Public Library of Science, vol. 11(12), pages 1-16, December.
    14. Geng Chen & Jun Xu & Asuka Inoue & Maximilian F. Schmidt & Chen Bai & Qiuyuan Lu & Peter Gmeiner & Zheng Liu & Yang Du, 2022. "Activation and allosteric regulation of the orphan GPR88-Gi1 signaling complex," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    15. Yuxia Qian & Jiening Wang & Linlin Yang & Yanru Liu & Lina Wang & Wei Liu & Yun Lin & Hong Yang & Lixin Ma & Sheng Ye & Shan Wu & Anna Qiao, 2022. "Activation and signaling mechanism revealed by GPR119-Gs complex structures," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    16. Jun Yu & Amit Kumar & Xuefeng Zhang & Charlotte Martin & Kevin Van holsbeeck & Pierre Raia & Antoine Koehl & Toon Laeremans & Jan Steyaert & Aashish Manglik & Steven Ballet & Andreas Boland & Miriam S, 2024. "Structural basis of μ-opioid receptor targeting by a nanobody antagonist," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    17. Meagan C Small & Pedro Lopes & Rodrigo B Andrade & Alexander D MacKerell Jr, 2013. "Impact of Ribosomal Modification on the Binding of the Antibiotic Telithromycin Using a Combined Grand Canonical Monte Carlo/Molecular Dynamics Simulation Approach," PLOS Computational Biology, Public Library of Science, vol. 9(6), pages 1-14, June.
    18. Wessel A. C. Burger & Vi Pham & Ziva Vuckovic & Alexander S. Powers & Jesse I. Mobbs & Yianni Laloudakis & Alisa Glukhova & Denise Wootten & Andrew B. Tobin & Patrick M. Sexton & Steven M. Paul & Chri, 2023. "Xanomeline displays concomitant orthosteric and allosteric binding modes at the M4 mAChR," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    19. Jacob D Feala & Jorge Cortes & Phillip M Duxbury & Andrew D McCulloch & Carlo Piermarocchi & Giovanni Paternostro, 2012. "Statistical Properties and Robustness of Biological Controller-Target Networks," PLOS ONE, Public Library of Science, vol. 7(1), pages 1-11, January.
    20. Xiao Teng & Sijia Chen & Yingying Nie & Peng Xiao & Xiao Yu & Zhenhua Shao & Sanduo Zheng, 2022. "Ligand recognition and biased agonism of the D1 dopamine receptor," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33156-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.