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Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

Author

Listed:
  • Maryam Khoshouei

    (Max Planck Institute of Biochemistry)

  • Mazdak Radjainia

    (The Clive and Vera Ramaciotti Centre for Cryo-EM, Monash University
    Present address: FEI, 5651 GG Eindhoven, The Netherlands)

  • Wolfgang Baumeister

    (Max Planck Institute of Biochemistry)

  • Radostin Danev

    (Max Planck Institute of Biochemistry)

Abstract

With the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM) have changed in a profound way. These cameras are superior to previous detectors in coping with the intrinsically low contrast and beam-induced motion of radiation-sensitive organic materials embedded in amorphous ice, and hence they have enabled the structure determination of many macromolecular assemblies to atomic or near-atomic resolution. Nevertheless, there are still limitations and one of them is the size of the target structure. Here, we report the use of a Volta phase plate in determining the structure of human haemoglobin (64 kDa) at 3.2 Å. Our results demonstrate that this method can be applied to complexes that are significantly smaller than those previously studied by conventional defocus-based approaches. Cryo-EM is now close to becoming a fast and cost-effective alternative to crystallography for high-resolution protein structure determination.

Suggested Citation

  • Maryam Khoshouei & Mazdak Radjainia & Wolfgang Baumeister & Radostin Danev, 2017. "Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate," Nature Communications, Nature, vol. 8(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms16099
    DOI: 10.1038/ncomms16099
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    Cited by:

    1. Zhiyuan Ding & Si Gao & Weina Fang & Chen Huang & Liqi Zhou & Xudong Pei & Xiaoguo Liu & Xiaoqing Pan & Chunhai Fan & Angus I. Kirkland & Peng Wang, 2022. "Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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