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Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome

Author

Listed:
  • Dariusz R. Kutyna

    (The Australian Wine Research Institute)

  • Cristobal A. Onetto

    (The Australian Wine Research Institute)

  • Thomas C. Williams

    (ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University)

  • Hugh D. Goold

    (ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
    Elizabeth Macarthur Agricultural Institute)

  • Ian T. Paulsen

    (ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University)

  • Isak S. Pretorius

    (ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
    Macquarie University)

  • Daniel L. Johnson

    (The Australian Wine Research Institute
    Macquarie University)

  • Anthony R. Borneman

    (The Australian Wine Research Institute
    The University of Adelaide)

Abstract

The Synthetic Yeast Genome Project (Sc2.0) represents the first foray into eukaryotic genome engineering and a framework for designing and building the next generation of industrial microbes. However, the laboratory strain S288c used lacks many of the genes that provide phenotypic diversity to industrial and environmental isolates. To address this shortcoming, we have designed and constructed a neo-chromosome that contains many of these diverse pan-genomic elements and which is compatible with the Sc2.0 design and test framework. The presence of this neo-chromosome provides phenotypic plasticity to the Sc2.0 parent strain, including expanding the range of utilizable carbon sources. We also demonstrate that the induction of programmable structural variation (SCRaMbLE) provides genetic diversity on which further adaptive gains could be selected. The presence of this neo-chromosome within the Sc2.0 backbone may therefore provide the means to adapt synthetic strains to a wider variety of environments, a process which will be vital to transitioning Sc2.0 from the laboratory into industrial applications.

Suggested Citation

  • Dariusz R. Kutyna & Cristobal A. Onetto & Thomas C. Williams & Hugh D. Goold & Ian T. Paulsen & Isak S. Pretorius & Daniel L. Johnson & Anthony R. Borneman, 2022. "Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31305-4
    DOI: 10.1038/s41467-022-31305-4
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    Cited by:

    1. Briardo Llorente & Thomas C. Williams & Hugh D. Goold & Isak S. Pretorius & Ian T. Paulsen, 2022. "Harnessing bioengineered microbes as a versatile platform for space nutrition," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    2. Alessandro L. V. Coradini & Christopher Ne Ville & Zachary A. Krieger & Joshua Roemer & Cara Hull & Shawn Yang & Daniel T. Lusk & Ian M. Ehrenreich, 2023. "Building synthetic chromosomes from natural DNA," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Charlotte Cautereels & Jolien Smets & Jonas De Saeger & Lloyd Cool & Yanmei Zhu & Anna Zimmermann & Jan Steensels & Anton Gorkovskiy & Thomas B. Jacobs & Kevin J. Verstrepen, 2024. "Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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