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Unlocking the functional potential of polyploid yeasts

Author

Listed:
  • Simone Mozzachiodi

    (Université Côte d’Azur, CNRS, INSERM, IRCAN
    Meiogenix)

  • Kristoffer Krogerus

    (VTT Technical Research Centre of Finland Ltd)

  • Brian Gibson

    (VTT Technical Research Centre of Finland Ltd
    Technical University)

  • Alain Nicolas

    (Université Côte d’Azur, CNRS, INSERM, IRCAN
    Meiogenix
    PSL Research University)

  • Gianni Liti

    (Université Côte d’Azur, CNRS, INSERM, IRCAN)

Abstract

Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market.

Suggested Citation

  • Simone Mozzachiodi & Kristoffer Krogerus & Brian Gibson & Alain Nicolas & Gianni Liti, 2022. "Unlocking the functional potential of polyploid yeasts," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30221-x
    DOI: 10.1038/s41467-022-30221-x
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    References listed on IDEAS

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    1. Jackson Peter & Matteo De Chiara & Anne Friedrich & Jia-Xing Yue & David Pflieger & Anders Bergström & Anastasie Sigwalt & Benjamin Barre & Kelle Freel & Agnès Llored & Corinne Cruaud & Karine Labadie, 2018. "Genome evolution across 1,011 Saccharomyces cerevisiae isolates," Nature, Nature, vol. 556(7701), pages 339-344, April.
    2. Jared Diamond, 2002. "Evolution, consequences and future of plant and animal domestication," Nature, Nature, vol. 418(6898), pages 700-707, August.
    3. Simone Mozzachiodi & Lorenzo Tattini & Agnes Llored & Agurtzane Irizar & Neža Škofljanc & Melania D’Angiolo & Matteo De Chiara & Benjamin P. Barré & Jia-Xing Yue & Angela Lutazi & Sophie Loeillet & Ra, 2021. "Aborting meiosis allows recombination in sterile diploid yeast hybrids," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    4. Shou-Fu Duan & Pei-Jie Han & Qi-Ming Wang & Wan-Qiu Liu & Jun-Yan Shi & Kuan Li & Xiao-Ling Zhang & Feng-Yan Bai, 2018. "The origin and adaptive evolution of domesticated populations of yeast from Far East Asia," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
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