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Building a eukaryotic chromosome arm by de novo design and synthesis

Author

Listed:
  • Shuangying Jiang

    (Chinese Academy of Sciences)

  • Zhouqing Luo

    (Chinese Academy of Sciences
    Xiamen University)

  • Jie Wu

    (Chinese Academy of Sciences)

  • Kang Yu

    (Chinese Academy of Sciences)

  • Shijun Zhao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zelin Cai

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Wenfei Yu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Hui Wang

    (Xiamen University)

  • Li Cheng

    (Chinese Academy of Sciences)

  • Zhenzhen Liang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Hui Gao

    (Chinese Academy of Sciences
    Shenzhen Bay Laboratory)

  • Marco Monti

    (University of Manchester)

  • Daniel Schindler

    (University of Manchester)

  • Linsen Huang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Cheng Zeng

    (Chinese Academy of Sciences)

  • Weimin Zhang

    (NYU Langone Health)

  • Chun Zhou

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yuanwei Tang

    (Chinese Academy of Sciences)

  • Tianyi Li

    (Chinese Academy of Sciences)

  • Yingxin Ma

    (Chinese Academy of Sciences)

  • Yizhi Cai

    (Chinese Academy of Sciences
    University of Manchester)

  • Jef D. Boeke

    (NYU Langone Health
    NYU Tandon School of Engineering)

  • Qiao Zhao

    (Chinese Academy of Sciences)

  • Junbiao Dai

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Agricultural Sciences)

Abstract

The genome of an organism is inherited from its ancestor and continues to evolve over time, however, the extent to which the current version could be altered remains unknown. To probe the genome plasticity of Saccharomyces cerevisiae, here we replace the native left arm of chromosome XII (chrXIIL) with a linear artificial chromosome harboring small sets of reconstructed genes. We find that as few as 12 genes are sufficient for cell viability, whereas 25 genes are required to recover the partial fitness defects observed in the 12-gene strain. Next, we demonstrate that these genes can be reconstructed individually using synthetic regulatory sequences and recoded open-reading frames with a “one-amino-acid-one-codon” strategy to remain functional. Finally, a synthetic neochromsome with the reconstructed genes is assembled which could substitute chrXIIL for viability. Together, our work not only highlights the high plasticity of yeast genome, but also illustrates the possibility of making functional eukaryotic chromosomes from entirely artificial sequences.

Suggested Citation

  • Shuangying Jiang & Zhouqing Luo & Jie Wu & Kang Yu & Shijun Zhao & Zelin Cai & Wenfei Yu & Hui Wang & Li Cheng & Zhenzhen Liang & Hui Gao & Marco Monti & Daniel Schindler & Linsen Huang & Cheng Zeng &, 2023. "Building a eukaryotic chromosome arm by de novo design and synthesis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43531-5
    DOI: 10.1038/s41467-023-43531-5
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    References listed on IDEAS

    as
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    2. Jessica S. Dymond & Sarah M. Richardson & Candice E. Coombes & Timothy Babatz & Héloïse Muller & Narayana Annaluru & William J. Blake & Joy W. Schwerzmann & Junbiao Dai & Derek L. Lindstrom & Annabel , 2011. "Synthetic chromosome arms function in yeast and generate phenotypic diversity by design," Nature, Nature, vol. 477(7365), pages 471-476, September.
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