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A genetic engineering strategy for editing near-infrared-II fluorophores

Author

Listed:
  • Rui Tian

    (Xiamen University)

  • Xin Feng

    (Xiamen University)

  • Long Wei

    (Xiamen University)

  • Daoguo Dai

    (Xiamen University)

  • Ying Ma

    (National Institutes of Health (NIH))

  • Haifeng Pan

    (Xiamen University)

  • Shengxiang Ge

    (Xiamen University)

  • Lang Bai

    (State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry)

  • Chaomin Ke

    (Xiamen University)

  • Yanlin Liu

    (Xiamen University)

  • Lixin Lang

    (National Institutes of Health (NIH))

  • Shoujun Zhu

    (State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry
    The First Hospital of Jilin University)

  • Haitao Sun

    (School of Physics and Materials Science, East China Normal University)

  • Yanbao Yu

    (J. Craig Venter Institute, 9714 Medical Center Drive
    University of Delaware, Newark)

  • Xiaoyuan Chen

    (Yong Loo Lin School of Medicine and Faculty of Engineering, National University of Singapore
    Yong Loo Lin School of Medicine, National University of Singapore
    NUS Center for Nanomedicine, Yong Loo Lin School of Medicine, National University of Singapore)

Abstract

The second near-infrared (NIR-II) window is a fundamental modality for deep-tissue in vivo imaging. However, it is challenging to synthesize NIR-II probes with high quantum yields (QYs), good biocompatibility, satisfactory pharmacokinetics, and tunable biological properties. Conventional long-wavelength probes, such as inorganic probes (which often contain heavy metal atoms in their scaffolds) and organic dyes (which contain large π-conjugated groups), exhibit poor biosafety, low QYs, and/or uncontrollable pharmacokinetic properties. Herein, we present a bioengineering strategy that can replace the conventional chemical synthesis methods for generating NIR-II contrast agents. We use a genetic engineering technique to obtain a series of albumin fragments and recombinant proteins containing one or multiple domains that form covalent bonds with chloro-containing cyanine dyes. These albumin variants protect the inserted dyes and remarkably enhance their brightness. The albumin variants can also be genetically edited to develop size-tunable complexes with precisely tailored pharmacokinetics. The proteins can also be conjugated to biofunctional molecules without impacting the complexed dyes. This combination of albumin mutants and clinically-used cyanine dyes can help widen the clinical application prospects of NIR-II fluorophores.

Suggested Citation

  • Rui Tian & Xin Feng & Long Wei & Daoguo Dai & Ying Ma & Haifeng Pan & Shengxiang Ge & Lang Bai & Chaomin Ke & Yanlin Liu & Lixin Lang & Shoujun Zhu & Haitao Sun & Yanbao Yu & Xiaoyuan Chen, 2022. "A genetic engineering strategy for editing near-infrared-II fluorophores," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30304-9
    DOI: 10.1038/s41467-022-30304-9
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    References listed on IDEAS

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    1. Yuanyuan Li & Zhaochong Cai & Shunjie Liu & Haoke Zhang & Sherman T. H. Wong & Jacky W. Y. Lam & Ryan T. K. Kwok & Jun Qian & Ben Zhong Tang, 2020. "Design of AIEgens for near-infrared IIb imaging through structural modulation at molecular and morphological levels," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    2. Peiyuan Wang & Yong Fan & Lingfei Lu & Lu Liu & Lingling Fan & Mengyao Zhao & Yang Xie & Congjian Xu & Fan Zhang, 2018. "NIR-II nanoprobes in-vivo assembly to improve image-guided surgery for metastatic ovarian cancer," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    3. Daniel Franke & Daniel K. Harris & Ou Chen & Oliver T. Bruns & Jessica A. Carr & Mark W. B. Wilson & Moungi G. Bawendi, 2016. "Continuous injection synthesis of indium arsenide quantum dots emissive in the short-wavelength infrared," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    4. Benhao Li & Mengyao Zhao & Lishuai Feng & Chaoran Dou & Suwan Ding & Gang Zhou & Lingfei Lu & Hongxin Zhang & Feiya Chen & Xiaomin Li & Guangfeng Li & Shichang Zhao & Chunyu Jiang & Yan Wang & Dongyua, 2020. "Organic NIR-II molecule with long blood half-life for in vivo dynamic vascular imaging," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
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    Cited by:

    1. Chao Mi & Xun Zhang & Chengyu Yang & Jianqun Wu & Xinxin Chen & Chenguang Ma & Sitong Wu & Zhichao Yang & Pengzhen Qiao & Yang Liu & Weijie Wu & Zhiyong Guo & Jiayan Liao & Jiajia Zhou & Ming Guan & C, 2023. "Bone disease imaging through the near-infrared-II window," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Jiajun Xu & Ningning Zhu & Yijing Du & Tianyang Han & Xue Zheng & Jia Li & Shoujun Zhu, 2024. "Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Wu, Zewen, 2024. "Are we in a bubble? Financial vulnerabilities in semiconductor, Web3, and genetic engineering markets," International Review of Economics & Finance, Elsevier, vol. 90(C), pages 32-44.

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