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Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination

Author

Listed:
  • Zhi-Zheng Wang

    (Central China Normal University)

  • Min-Jie Cao

    (Southern University of Science and Technology)

  • Junjie Yan

    (Huazhong Agricultural University)

  • Jin Dong

    (Central China Normal University)

  • Mo-Xian Chen

    (Guizhou University)

  • Jing-Fang Yang

    (Central China Normal University)

  • Jian-Hong Li

    (Guizhou University)

  • Rui-Ning Ying

    (Central China Normal University)

  • Yang-Yang Gao

    (Guizhou University)

  • Li Li

    (Huazhong Agricultural University)

  • Ya-Nan Leng

    (Nanjing Forestry University)

  • Yuan Tian

    (Shandong Agricultural University)

  • Kamalani Achala H. Hewage

    (Central China Normal University)

  • Rong-Jie Pei

    (Central China Normal University)

  • Zhi-You Huang

    (Central China Normal University)

  • Ping Yin

    (Huazhong Agricultural University)

  • Jian-Kang Zhu

    (Southern University of Science and Technology)

  • Ge-Fei Hao

    (Central China Normal University
    Guizhou University)

  • Guang-Fu Yang

    (Central China Normal University)

Abstract

Abscisic acid (ABA) is the primary preventing factor of seed germination, which is crucial to plant survival and propagation. ABA-induced seed germination inhibition is mainly mediated by the dimeric PYR/PYL/RCAR (PYLs) family members. However, little is known about the relevance between dimeric stability of PYLs and seed germination. Here, we reveal that stabilization of PYL dimer can relieve ABA-induced inhibition of seed germination using chemical genetic approaches. Di-nitrobensulfamide (DBSA), a computationally designed chemical probe, yields around ten-fold improvement in receptor affinity relative to ABA. DBSA reverses ABA-induced inhibition of seed germination mainly through dimeric receptors and recovers the expression of ABA-responsive genes. DBSA maintains PYR1 in dimeric state during protein oligomeric state experiment. X-ray crystallography shows that DBSA targets a pocket in PYL dimer interface and may stabilize PYL dimer by forming hydrogen networks. Our results illustrate the potential of PYL dimer stabilization in preventing ABA-induced seed germination inhibition.

Suggested Citation

  • Zhi-Zheng Wang & Min-Jie Cao & Junjie Yan & Jin Dong & Mo-Xian Chen & Jing-Fang Yang & Jian-Hong Li & Rui-Ning Ying & Yang-Yang Gao & Li Li & Ya-Nan Leng & Yuan Tian & Kamalani Achala H. Hewage & Rong, 2024. "Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52426-y
    DOI: 10.1038/s41467-024-52426-y
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    References listed on IDEAS

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