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In vitro reconstitution of an abscisic acid signalling pathway

Author

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  • Hiroaki Fujii

    (University of California at Riverside, Riverside, California 92521, USA
    Center for Plant Stress Genomics and Technology, 4700 King Abdullah University of Science and Technology)

  • Viswanathan Chinnusamy

    (University of California at Riverside, Riverside, California 92521, USA
    Center for Plant Stress Genomics and Technology, 4700 King Abdullah University of Science and Technology)

  • Americo Rodrigues

    (Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, ES-46022 Valencia, Spain)

  • Silvia Rubio

    (Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, ES-46022 Valencia, Spain)

  • Regina Antoni

    (Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, ES-46022 Valencia, Spain)

  • Sang-Youl Park

    (University of California at Riverside, Riverside, California 92521, USA)

  • Sean R. Cutler

    (University of California at Riverside, Riverside, California 92521, USA)

  • Jen Sheen

    (Massachusetts General Hospital, Boston, Massachusetts 02114, USA)

  • Pedro L. Rodriguez

    (Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, ES-46022 Valencia, Spain)

  • Jian-Kang Zhu

    (University of California at Riverside, Riverside, California 92521, USA
    Center for Plant Stress Genomics and Technology, 4700 King Abdullah University of Science and Technology)

Abstract

Abscisic acid signalling reconstituted in vitro The phytohormone abscisic acid (ABA) plays an important role in several physiological responses such as stomatal conductance and seed dormancy and also in protecting plants against stress conditions such as drought and cold. Recently a family of proteins known as PYR/PYL/RCAR were identified as ABA receptors, but details of the signalling pathway downstream of ABA binding remained unclear. In this paper, Zhu and colleagues reconstitute the ABA-mediated signalling in vitro and test key observations in vivo. This is the first reported reconstitution of a plant hormone signalling pathway.

Suggested Citation

  • Hiroaki Fujii & Viswanathan Chinnusamy & Americo Rodrigues & Silvia Rubio & Regina Antoni & Sang-Youl Park & Sean R. Cutler & Jen Sheen & Pedro L. Rodriguez & Jian-Kang Zhu, 2009. "In vitro reconstitution of an abscisic acid signalling pathway," Nature, Nature, vol. 462(7273), pages 660-664, December.
    • Handle: RePEc:nat:nature:v:462:y:2009:i:7273:d:10.1038_nature08599
    DOI: 10.1038/nature08599
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    Citations

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    Cited by:

    1. Jia Zhou & Qinli Hu & Xinlong Xiao & Deqiang Yao & Shenghong Ge & Jin Ye & Haojie Li & Rujie Cai & Renyang Liu & Fangang Meng & Chao Wang & Jian-Kang Zhu & Mingguang Lei & Weiman Xing, 2021. "Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Shen Huang & Chunli Wang & Zixuan Ding & Yaqian Zhao & Jing Dai & Jia Li & Haining Huang & Tongkai Wang & Min Zhu & Mingfeng Feng & Yinghua Ji & Zhongkai Zhang & Xiaorong Tao, 2024. "A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Zhang You & Shiyuan Guo & Qiao Li & Yanjun Fang & Panpan Huang & Chuanfeng Ju & Cun Wang, 2023. "The CBL1/9-CIPK1 calcium sensor negatively regulates drought stress by phosphorylating the PYLs ABA receptor," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Benjamin J. M. Tremblay & Cristina P. Santini & Yajiao Cheng & Xue Zhang & Stefanie Rosa & Julia I. Qüesta, 2024. "Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    5. Zhi-Zheng Wang & Min-Jie Cao & Junjie Yan & Jin Dong & Mo-Xian Chen & Jing-Fang Yang & Jian-Hong Li & Rui-Ning Ying & Yang-Yang Gao & Li Li & Ya-Nan Leng & Yuan Tian & Kamalani Achala H. Hewage & Rong, 2024. "Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    6. Junyao Lu & Ning Li & Gaojian Li & Ziang Tian & Lianping Shi & Yan Wang & Yingao Cai & Kaiyuan Zhang & Wanting Sun & Danyang Wang & Jinxin Lin & Jinguang Huang & Changai Wu & Kang Yan & Shizhong Zhang, 2024. "N-glycosylation of SnRK2s affects NADPH maintenance in peroxisomes during prolonged ABA signalling," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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