IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26736-4.html
   My bibliography  Save this article

Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

Author

Listed:
  • Che-Hang Yu

    (University of California)

  • Jeffrey N. Stirman

    (LifeCanvas Technologies)

  • Yiyi Yu

    (University of California)

  • Riichiro Hira

    (University of California)

  • Spencer L. Smith

    (University of California)

Abstract

Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm2, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

Suggested Citation

  • Che-Hang Yu & Jeffrey N. Stirman & Yiyi Yu & Riichiro Hira & Spencer L. Smith, 2021. "Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26736-4
    DOI: 10.1038/s41467-021-26736-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26736-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26736-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Mitchell Clough & Ichun Anderson Chen & Seong-Wook Park & Allison M. Ahrens & Jeffrey N. Stirman & Spencer L. Smith & Jerry L. Chen, 2021. "Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    2. Tsai-Wen Chen & Trevor J. Wardill & Yi Sun & Stefan R. Pulver & Sabine L. Renninger & Amy Baohan & Eric R. Schreiter & Rex A. Kerr & Michael B. Orger & Vivek Jayaraman & Loren L. Looger & Karel Svobod, 2013. "Ultrasensitive fluorescent proteins for imaging neuronal activity," Nature, Nature, vol. 499(7458), pages 295-300, July.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Maximilian Hoffmann & Jörg Henninger & Johannes Veith & Lars Richter & Benjamin Judkewitz, 2023. "Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Aniruddha Das & Sarah Holden & Julie Borovicka & Jacob Icardi & Abigail O’Niel & Ariel Chaklai & Davina Patel & Rushik Patel & Stefanie Kaech Petrie & Jacob Raber & Hod Dana, 2023. "Large-scale recording of neuronal activity in freely-moving mice at cellular resolution," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Johannes Friedrich & Pengcheng Zhou & Liam Paninski, 2017. "Fast online deconvolution of calcium imaging data," PLOS Computational Biology, Public Library of Science, vol. 13(3), pages 1-26, March.
    3. Jen-Chun Hsiang & Ning Shen & Florentina Soto & Daniel Kerschensteiner, 2024. "Distributed feature representations of natural stimuli across parallel retinal pathways," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    4. Omer Mano & Damon A Clark, 2017. "Graphics Processing Unit-Accelerated Code for Computing Second-Order Wiener Kernels and Spike-Triggered Covariance," PLOS ONE, Public Library of Science, vol. 12(1), pages 1-11, January.
    5. Zengpeng Han & Nengsong Luo & Wenyu Ma & Xiaodong Liu & Yuxiang Cai & Jiaxin Kou & Jie Wang & Lei Li & Siqi Peng & Zihong Xu & Wen Zhang & Yuxiang Qiu & Yang Wu & Chaohui Ye & Kunzhang Lin & Fuqiang X, 2023. "AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    6. Philipp Berens & Jeremy Freeman & Thomas Deneux & Nikolay Chenkov & Thomas McColgan & Artur Speiser & Jakob H Macke & Srinivas C Turaga & Patrick Mineault & Peter Rupprecht & Stephan Gerhard & Rainer , 2018. "Community-based benchmarking improves spike rate inference from two-photon calcium imaging data," PLOS Computational Biology, Public Library of Science, vol. 14(5), pages 1-13, May.
    7. Jilt Sebastian & Mriganka Sur & Hema A Murthy & Mathew Magimai-Doss, 2021. "Signal-to-signal neural networks for improved spike estimation from calcium imaging data," PLOS Computational Biology, Public Library of Science, vol. 17(3), pages 1-19, March.
    8. Richard F Betzel & Katherine C Wood & Christopher Angeloni & Maria Neimark Geffen & Danielle S Bassett, 2019. "Stability of spontaneous, correlated activity in mouse auditory cortex," PLOS Computational Biology, Public Library of Science, vol. 15(12), pages 1-25, December.
    9. Jina Yun & Simon Hansen & Otto Morris & David T. Madden & Clare Peters Libeu & Arjun J. Kumar & Cameron Wehrfritz & Aaron H. Nile & Yingnan Zhang & Lijuan Zhou & Yuxin Liang & Zora Modrusan & Michelle, 2023. "Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    10. Guillaume Viejo & Thomas Cortier & Adrien Peyrache, 2018. "Brain-state invariant thalamo-cortical coordination revealed by non-linear encoders," PLOS Computational Biology, Public Library of Science, vol. 14(3), pages 1-25, March.
    11. Liang Shi & Xiaoxi Fu & Shen Gui & Tong Wan & Junjie Zhuo & Jinling Lu & Pengcheng Li, 2024. "Global spatiotemporal synchronizing structures of spontaneous neural activities in different cell types," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    12. Yuanlong Zhang & Xiaofei Song & Jiachen Xie & Jing Hu & Jiawei Chen & Xiang Li & Haiyu Zhang & Qiqun Zhou & Lekang Yuan & Chui Kong & Yibing Shen & Jiamin Wu & Lu Fang & Qionghai Dai, 2023. "Large depth-of-field ultra-compact microscope by progressive optimization and deep learning," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    13. Jeffrey P Nguyen & Ashley N Linder & George S Plummer & Joshua W Shaevitz & Andrew M Leifer, 2017. "Automatically tracking neurons in a moving and deforming brain," PLOS Computational Biology, Public Library of Science, vol. 13(5), pages 1-19, May.
    14. Franziska Bierbuesse & Anaïs C. Bourges & Vincent Gielen & Viola Mönkemöller & Wim Vandenberg & Yi Shen & Johan Hofkens & Pieter Vanden Berghe & Robert E. Campbell & Benjamien Moeyaert & Peter Dedecke, 2022. "Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    15. Johannes Friedrich & Weijian Yang & Daniel Soudry & Yu Mu & Misha B Ahrens & Rafael Yuste & Darcy S Peterka & Liam Paninski, 2017. "Multi-scale approaches for high-speed imaging and analysis of large neural populations," PLOS Computational Biology, Public Library of Science, vol. 13(8), pages 1-24, August.
    16. Jimin Wu & Yuzhi Chen & Ashok Veeraraghavan & Eyal Seidemann & Jacob T. Robinson, 2024. "Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    17. Sophie Aimon & Takeo Katsuki & Tongqiu Jia & Logan Grosenick & Michael Broxton & Karl Deisseroth & Terrence J Sejnowski & Ralph J Greenspan, 2019. "Fast near-whole–brain imaging in adult Drosophila during responses to stimuli and behavior," PLOS Biology, Public Library of Science, vol. 17(2), pages 1-31, February.
    18. Hyung Chul Lee & Nidia M. M. Oliveira & Cato Hastings & Peter Baillie-Benson & Adam A. Moverley & Hui-Chun Lu & Yi Zheng & Elise L. Wilby & Timothy T. Weil & Karen M. Page & Jianping Fu & Naomi Moris , 2024. "Regulation of long-range BMP gradients and embryonic polarity by propagation of local calcium-firing activity," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    19. Roman Dvorkin & Noam E Ziv, 2016. "Relative Contributions of Specific Activity Histories and Spontaneous Processes to Size Remodeling of Glutamatergic Synapses," PLOS Biology, Public Library of Science, vol. 14(10), pages 1-33, October.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26736-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.