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Taxonomic and Biochemical Characterization of Microalga Graesiella emersonii GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts

Author

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  • Nam Seon Kang

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Kichul Cho

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Sung Min An

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Eun Song Kim

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Hyunji Ki

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Chung Hyeon Lee

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Grace Choi

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Ji Won Hong

    (Department of Hydrogen and Renewable Energy, Kyungpook National University, Daegu 41566, Republic of Korea
    Advanced Bio-Resource Research Center, Kyungpook National University, Daegu 41566, Republic of Korea)

Abstract

Graesiella emersonii is a commercially exploitable source of bioactive compounds and biofuels with potential applications in microalgae-based industries. Despite this, little taxonomical information is available. Therefore, proper identification and characterization are needed for the sustainable utilization of isolated microalgae. In this study, an axenically isolated unicellular green alga from the Geumgang Estuary, Korea was investigated for its morphological, molecular, and biochemical characteristics. The morphological characteristics were typical of G. emersonii. Molecular phylogenetic analysis of the 18S rDNA sequence verified that the isolate belonged to G. emersonii and was subsequently named G. emersonii GEGS21. It was isolated from brackish water, and its optimal growth temperature, salinity, and light intensity were at 28–32 °C, 0 M NaCl, and 130–160 µmol m −2 s −1 , respectively. The strain thrived over a range of temperatures (5–40 °C) and withstood up to 0.5 M NaCl. The isolate was rich in omega-6 linoleic acid (C 18:2 n-6, 26.3%) and palmitic acid (C 16:0 , 27.5%). The fuel quality properties were determined, and biodiesel from GEGS21 could be used as a biodiesel blend. Value-added carotenoids lutein (1.5 mg g −1 dry cell weight, DCW) and neoxanthin (1.2 mg g −1 DCW) were biosynthesized as accessory pigments by this microalga. The biomass of this microalga may serve as feedstock for biodiesel production as well as producing valuable ω-6 and carotenoids.

Suggested Citation

  • Nam Seon Kang & Kichul Cho & Sung Min An & Eun Song Kim & Hyunji Ki & Chung Hyeon Lee & Grace Choi & Ji Won Hong, 2022. "Taxonomic and Biochemical Characterization of Microalga Graesiella emersonii GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts," Energies, MDPI, vol. 15(22), pages 1-24, November.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:22:p:8725-:d:978462
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    References listed on IDEAS

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