IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v179y2021icp2004-2015.html
   My bibliography  Save this article

De novo transcriptomic analysis of Graesiella emersonii NC-M1 reveals differential genes expression in cell proliferation and lipid production under glucose and salt supplemented condition

Author

Listed:
  • Mandal, Madan Kumar
  • Chaurasia, Neha

Abstract

In the present study, under single-stage cultivation (mixotrophic mode), the effect of glucose and salt addition on biomass growth, lipid productivity, and FAME profiles in Graesiella emersonii NC-M1 were performed and analyzed. Under this condition (1.75 g/L glucose and 0.3M NaCl addition), a 3.3-, 4.65- and 4.56-fold higher biomass concentration, lipid concentration, and lipid productivity, respectively, was recorded compared to the control. To understand mechanisms underlying higher biomass and lipid production, de novo transcriptome analysis was performed. The transcriptome analysis revealed that the genes responsible for cell proliferation (transcription factor Dp-1, CDC48E) and NADPH production were significantly up-regulated under treated condition. Further, starch synthase showed significant down-regulation that illustrates a shift of carbon-flux for the biosynthesis of lipid. This study unravels the mechanisms influencing rapid growth and lipid accumulation under treated condition and provides new genetic targets for strain improvement of biodiesel-producing microalgae.

Suggested Citation

  • Mandal, Madan Kumar & Chaurasia, Neha, 2021. "De novo transcriptomic analysis of Graesiella emersonii NC-M1 reveals differential genes expression in cell proliferation and lipid production under glucose and salt supplemented condition," Renewable Energy, Elsevier, vol. 179(C), pages 2004-2015.
  • Handle: RePEc:eee:renene:v:179:y:2021:i:c:p:2004-2015
    DOI: 10.1016/j.renene.2021.07.141
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148121011538
    Download Restriction: Full text for ScienceDirect subscribers only

    File URL: https://libkey.io/10.1016/j.renene.2021.07.141?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Morais, Keli C.C. & Conceição, Daniele & Vargas, José V.C. & Mitchell, David A. & Mariano, André B. & Ordonez, Juan C. & Galli-Terasawa, Lygia Vitoria & Kava, Vanessa M., 2021. "Enhanced microalgae biomass and lipid output for increased biodiesel productivity," Renewable Energy, Elsevier, vol. 163(C), pages 138-145.
    2. Poh, Zhia Lerc & Amalina Kadir, Wan Nadiah & Lam, Man Kee & Uemura, Yoshimitsu & Suparmaniam, Uganeeswary & Lim, Jun Wei & Show, Pau Loke & Lee, Keat Tong, 2020. "The effect of stress environment towards lipid accumulation in microalgae after harvesting," Renewable Energy, Elsevier, vol. 154(C), pages 1083-1091.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Nam Seon Kang & Kichul Cho & Sung Min An & Eun Song Kim & Hyunji Ki & Chung Hyeon Lee & Grace Choi & Ji Won Hong, 2022. "Taxonomic and Biochemical Characterization of Microalga Graesiella emersonii GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts," Energies, MDPI, vol. 15(22), pages 1-24, November.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Lim, Juin Yau & Teng, Sin Yong & How, Bing Shen & Nam, KiJeon & Heo, SungKu & Máša, Vítězslav & Stehlík, Petr & Yoo, Chang Kyoo, 2022. "From microalgae to bioenergy: Identifying optimally integrated biorefinery pathways and harvest scheduling under uncertainties in predicted climate," Renewable and Sustainable Energy Reviews, Elsevier, vol. 168(C).
    2. Leong, Wai Hong & Kiatkittipong, Worapon & Lam, Man Kee & Khoo, Kuan Shiong & Show, Pau Loke & Mohamad, Mardawani & Chong, Siewhui & Abdurrahman, Muslim & Lim, Jun Wei, 2022. "Dual nutrient heterogeneity modes in a continuous flow photobioreactor for optimum nitrogen assimilation to produce microalgal biodiesel," Renewable Energy, Elsevier, vol. 184(C), pages 443-451.
    3. Sultana, N. & Hossain, S.M. Zakir & Albalooshi, H.A. & Chrouf, S.M.B. & AlNajar, I.A. & Alhindi, K.R. & AlMofeez, K.A. & Razzak, S.A. & Hossain, M.M., 2021. "Soft computing modeling and multiresponse optimization for production of microalgal biomass and lipid as bioenergy feedstock," Renewable Energy, Elsevier, vol. 178(C), pages 1020-1033.
    4. Yin, Zhihong & Chu, Ruoyu & Zhu, Liandong & Li, Shuangxi & Mo, Fan & Hu, Dan & Liu, Chenchen, 2021. "Application of chitosan-based flocculants to harvest microalgal biomass for biofuel production: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 145(C).
    5. Shokravi, Zahra & Shokravi, Hoofar & Atabani, A.E. & Lau, Woei Jye & Chyuan, Ong Hwai & Ismail, Ahmad Fauzi, 2022. "Impacts of the harvesting process on microalgae fatty acid profiles and lipid yields: Implications for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 161(C).
    6. Kurt, Ç. & Ekinci, K. & Uysal, Ö., 2022. "The effect of LEDs on the growth and fatty acid composition of Botryococcus braunii," Renewable Energy, Elsevier, vol. 186(C), pages 66-73.
    7. Abomohra, Abd El-Fatah & Eladel, Hamed & Mohammed, Soha, 2022. "Dual use of a local Protosiphon isolate BENHA2020 for biodiesel production and antioxidant activity of lipid-free biomass: A novel biorefinery approach for biomass valorization," Renewable Energy, Elsevier, vol. 184(C), pages 1104-1111.
    8. Visva Bharati Barua & Mariya Munir, 2021. "A Review on Synchronous Microalgal Lipid Enhancement and Wastewater Treatment," Energies, MDPI, vol. 14(22), pages 1-20, November.
    9. Behera, Bunushree & Unpaprom, Yuwalee & Ramaraj, Rameshprabu & Maniam, Gaanty Pragas & Govindan, Natanamurugaraj & Paramasivan, Balasubramanian, 2021. "Integrated biomolecular and bioprocess engineering strategies for enhancing the lipid yield from microalgae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 148(C).
    10. Thurakit, Theera & Pathom-aree, Wasu & Pumas, Chayakorn & Brocklehurst, Thanyanan Wannathong & Pekkoh, Jeeraporn & Srinuanpan, Sirasit, 2022. "High-efficiency production of biomass and biofuel under two-stage cultivation of a stable microalga Botryococcus braunii mutant generated by ethyl methanesulfonate-induced mutation," Renewable Energy, Elsevier, vol. 198(C), pages 176-188.
    11. Zahra Shokravi & Hoofar Shokravi & Ong Hwai Chyuan & Woei Jye Lau & Seyed Saeid Rahimian Koloor & Michal Petrů & Ahmad Fauzi Ismail, 2020. "Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review," Sustainability, MDPI, vol. 12(21), pages 1-28, October.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:179:y:2021:i:c:p:2004-2015. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.