IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v11y2018i4p886-d140447.html
   My bibliography  Save this article

Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis

Author

Listed:
  • Rafał Łukajtis

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Piotr Rybarczyk

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Karolina Kucharska

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Donata Konopacka-Łyskawa

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Edyta Słupek

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Katarzyna Wychodnik

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Marian Kamiński

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

Abstract

Pre-treatment is a significant step in the production of second-generation biofuels from waste lignocellulosic materials. Obtaining biofuels as a result of fermentation processes requires appropriate pre-treatment conditions ensuring the highest possible degree of saccharification of the feed material. An influence of the following process parameters were investigated for alkaline pre-treatment of Salix viminalis L.: catalyst concentration (NaOH), temperature, pre-treatment time and granulation. For this purpose, experiments were carried out in accordance to the Box-Behnken design for four factors. In the saccharification process of the pre-treated biomass, cellulolytic enzymes immobilized on diatomaceous earth were used. Based on the obtained results, a mathematical model for the optimal conditions of alkaline pre-treatment prediction is proposed. The optimal conditions of alkaline pre-treatment are established as follows: granulation 0.75 mm, catalyst concentration 7%, pre-treatment time 6 h and temperature 65 °C if the saccharification efficiency and cost analysis are considered. An influence of the optimized pre-treatment on both the chemical composition and structural changes for six various lignocellulosic materials (energetic willow, energetic poplar, beech, triticale, meadow grass, corncobs) was investigated. SEM images of raw and pre-treated biomass samples are included in order to follow the changes in the biomass structure during hydrolysis.

Suggested Citation

  • Rafał Łukajtis & Piotr Rybarczyk & Karolina Kucharska & Donata Konopacka-Łyskawa & Edyta Słupek & Katarzyna Wychodnik & Marian Kamiński, 2018. "Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis," Energies, MDPI, vol. 11(4), pages 1-27, April.
    • Handle: RePEc:gam:jeners:v:11:y:2018:i:4:p:886-:d:140447
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/11/4/886/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/11/4/886/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Abdi Hanra Sebayang & Masjuki Haji Hassan & Hwai Chyuan Ong & Surya Dharma & Arridina Susan Silitonga & Fitranto Kusumo & Teuku Meurah Indra Mahlia & Aditiya Harjon Bahar, 2017. "Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology," Energies, MDPI, vol. 10(1), pages 1-13, January.
    2. Zhang, Qi & Zhang, Pengfei & Pei, Z.J. & Wang, Donghai, 2013. "Relationships between cellulosic biomass particle size and enzymatic hydrolysis sugar yield: Analysis of inconsistent reports in the literature," Renewable Energy, Elsevier, vol. 60(C), pages 127-136.
    3. Wang, Tiejun & Li, Kai & Liu, Qiying & Zhang, Qing & Qiu, Songbai & Long, Jinxing & Chen, Lungang & Ma, Longlong & Zhang, Qi, 2014. "Aviation fuel synthesis by catalytic conversion of biomass hydrolysate in aqueous phase," Applied Energy, Elsevier, vol. 136(C), pages 775-780.
    4. Marwa M. El-Dalatony & El-Sayed Salama & Mayur B. Kurade & Sedky H. A. Hassan & Sang-Eun Oh & Sunjoon Kim & Byong-Hun Jeon, 2017. "Utilization of Microalgal Biofractions for Bioethanol, Higher Alcohols, and Biodiesel Production: A Review," Energies, MDPI, vol. 10(12), pages 1-19, December.
    5. Rafał Łukajtis & Karolina Kucharska & Iwona Hołowacz & Piotr Rybarczyk & Katarzyna Wychodnik & Edyta Słupek & Paulina Nowak & Marian Kamiński, 2018. "Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation," Energies, MDPI, vol. 11(3), pages 1-24, March.
    6. Gianluca Cavalaglio & Mattia Gelosia & Silvia D’Antonio & Andrea Nicolini & Anna Laura Pisello & Marco Barbanera & Franco Cotana, 2016. "Lignocellulosic Ethanol Production from the Recovery of Stranded Driftwood Residues," Energies, MDPI, vol. 9(8), pages 1-10, August.
    7. Hoang-Tuong Nguyen Hao & Obulisamy Parthiba Karthikeyan & Kirsten Heimann, 2015. "Bio-Refining of Carbohydrate-Rich Food Waste for Biofuels," Energies, MDPI, vol. 8(7), pages 1-15, June.
    8. Manoj Kandasamy & Ihsan Hamawand & Leslie Bowtell & Saman Seneweera & Sayan Chakrabarty & Talal Yusaf & Zaidoon Shakoor & Sattar Algayyim & Friederike Eberhard, 2017. "Investigation of Ethanol Production Potential from Lignocellulosic Material without Enzymatic Hydrolysis Using the Ultrasound Technique," Energies, MDPI, vol. 10(1), pages 1-12, January.
    9. Giorgos Markou & Irini Angelidaki & Elias Nerantzis & Dimitris Georgakakis, 2013. "Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) p latensis," Energies, MDPI, vol. 6(8), pages 1-14, August.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Karolina Kucharska & Patrycja Makoś-Chełstowska & Edyta Słupek & Jacek Gębicki, 2021. "Management of Dark Fermentation Broth via Bio Refining and Photo Fermentation," Energies, MDPI, vol. 14(19), pages 1-16, October.
    2. Mariana Abreu & Luís Silva & Belina Ribeiro & Alice Ferreira & Luís Alves & Susana M. Paixão & Luísa Gouveia & Patrícia Moura & Florbela Carvalheiro & Luís C. Duarte & Ana Luisa Fernando & Alberto Rei, 2022. "Low Indirect Land Use Change (ILUC) Energy Crops to Bioenergy and Biofuels—A Review," Energies, MDPI, vol. 15(12), pages 1-68, June.
    3. Vitor B. Furlong & Luciano J. Corrêa & Roberto C. Giordano & Marcelo P. A. Ribeiro, 2019. "Fuzzy-Enhanced Modeling of Lignocellulosic Biomass Enzymatic Saccharification," Energies, MDPI, vol. 12(11), pages 1-17, June.
    4. Vincent Oriez & Jérôme Peydecastaing & Pierre-Yves Pontalier, 2020. "Lignocellulosic Biomass Mild Alkaline Fractionation and Resulting Extract Purification Processes: Conditions, Yields, and Purities," Clean Technol., MDPI, vol. 2(1), pages 1-25, February.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Rodica Niculescu & Adrian Clenci & Victor Iorga-Siman, 2019. "Review on the Use of Diesel–Biodiesel–Alcohol Blends in Compression Ignition Engines," Energies, MDPI, vol. 12(7), pages 1-41, March.
    2. Atsonios, Konstantinos & Kougioumtzis, Michael-Alexander & D. Panopoulos, Kyriakos & Kakaras, Emmanuel, 2015. "Alternative thermochemical routes for aviation biofuels via alcohols synthesis: Process modeling, techno-economic assessment and comparison," Applied Energy, Elsevier, vol. 138(C), pages 346-366.
    3. Chen Zhang & Lei Luo & Wei Chen & Fei Yang & Gang Luo & Junming Xu, 2022. "Experimental Investigation on the Performance of an Aviation Piston Engine Fueled with Bio-Jet Fuel Prepared via Thermochemical Conversion of Triglyceride," Energies, MDPI, vol. 15(9), pages 1-13, April.
    4. Li, Yuping & Huang, Xiaoming & Zhang, Qian & Chen, Lungang & Zhang, Xinghua & Wang, Tiejun & Ma, Longlong, 2015. "Hydrogenation and hydrodeoxygenation of difurfurylidene acetone to liquid alkanes over Raney Ni and the supported Pt catalysts," Applied Energy, Elsevier, vol. 160(C), pages 990-998.
    5. Tuan Hoang, Anh & Viet Pham, Van, 2021. "2-Methylfuran (MF) as a potential biofuel: A thorough review on the production pathway from biomass, combustion progress, and application in engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 148(C).
    6. Manju Dhakad Tanwar & Felipe Andrade Torres & Ali Mubarak Alqahtani & Pankaj Kumar Tanwar & Yashas Bhand & Omid Doustdar, 2023. "Promising Bioalcohols for Low-Emission Vehicles," Energies, MDPI, vol. 16(2), pages 1-22, January.
    7. Eun-Young Park & Jung-Kyu Park, 2021. "Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass," Energies, MDPI, vol. 14(23), pages 1-9, December.
    8. Beata Brzychczyk & Tomasz Hebda & Norbert Pedryc, 2020. "The Influence of Artificial Lighting Systems on the Cultivation of Algae: The Example of Chlorella vulgaris," Energies, MDPI, vol. 13(22), pages 1-14, November.
    9. Kyriakou, Maria & Patsalou, Maria & Xiaris, Nikolas & Tsevis, Athanasios & Koutsokeras, Loukas & Constantinides, Georgios & Koutinas, Michalis, 2020. "Enhancing bioproduction and thermotolerance in Saccharomyces cerevisiae via cell immobilization on biochar: Application in a citrus peel waste biorefinery," Renewable Energy, Elsevier, vol. 155(C), pages 53-64.
    10. Francisco Anguebes-Franseschi & Mohamed Abatal & Ali Bassam & Mauricio A. Escalante Soberanis & Oscar May Tzuc & Lauro Bucio-Galindo & Atl Victor Cordova Quiroz & Claudia Alejandra Aguilar Ucan & Migu, 2018. "Esterification Optimization of Crude African Palm Olein Using Response Surface Methodology and Heterogeneous Acid Catalysis," Energies, MDPI, vol. 11(1), pages 1-15, January.
    11. Qi Zhang & Zhenzhen Shi & Pengfei Zhang & Meng Zhang & Zhichao Li & Xi Chen & Jiping Zhou, 2018. "Ultrasonic-Assisted Pelleting of Sorghum Stalk: Predictive Models for Pellet Density and Durability Using Multiple Response Surface Methodology," Energies, MDPI, vol. 11(5), pages 1-18, May.
    12. Tran Thi Giang & Siriporn Lunprom & Qiang Liao & Alissara Reungsang & Apilak Salakkam, 2019. "Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)," Energies, MDPI, vol. 12(5), pages 1-14, March.
    13. Sattar Jabbar Murad Algayyim & Talal Yusaf & Naseer H. Hamza & Andrew P. Wandel & I. M. Rizwanul Fattah & Mohamd Laimon & S. M. Ashrafur Rahman, 2022. "Sugarcane Biomass as a Source of Biofuel for Internal Combustion Engines (Ethanol and Acetone-Butanol-Ethanol): A Review of Economic Challenges," Energies, MDPI, vol. 15(22), pages 1-17, November.
    14. Yu, Kai Ling & Chen, Wei-Hsin & Sheen, Herng-Kuang & Chang, Jo-Shu & Lin, Chih-Sheng & Ong, Hwai Chyuan & Show, Pau Loke & Ng, Eng-Poh & Ling, Tau Chuan, 2020. "Production of microalgal biochar and reducing sugar using wet torrefaction with microwave-assisted heating and acid hydrolysis pretreatment," Renewable Energy, Elsevier, vol. 156(C), pages 349-360.
    15. Ngamsirisomsakul, Marika & Reungsang, Alissara & Liao, Qiang & Kongkeitkajorn, Mallika Boonmee, 2019. "Enhanced bio-ethanol production from Chlorella sp. biomass by hydrothermal pretreatment and enzymatic hydrolysis," Renewable Energy, Elsevier, vol. 141(C), pages 482-492.
    16. Witold Ilewicz & Piotr Skupin & Dariusz Choiński & Wojciech Błotnicki & Zdzisław Bielecki, 2020. "On-Line Estimation of the Ultrasonic Power in a Continuous Flow Sonochemical Reactor," Energies, MDPI, vol. 13(11), pages 1-10, June.
    17. Balasubramani Ravindran & Sanjay Kumar Gupta & Won-Mo Cho & Jung Kon Kim & Sang Ryong Lee & Kwang-Hwa Jeong & Dong Jun Lee & Hee-Chul Choi, 2016. "Microalgae Potential and Multiple Roles—Current Progress and Future Prospects—An Overview," Sustainability, MDPI, vol. 8(12), pages 1-16, November.
    18. Maurizio Carlini & Sonia Castellucci & Guomin Sun & Jinsong Leng & Carlo Cattani & Alessandro Cardarelli, 2018. "A Wavelet-Based Optimization Method for Biofuel Production," Energies, MDPI, vol. 11(2), pages 1-17, February.
    19. Ong, Victor Zhenquan & Wu, Ta Yeong, 2020. "An application of ultrasonication in lignocellulosic biomass valorisation into bio-energy and bio-based products," Renewable and Sustainable Energy Reviews, Elsevier, vol. 132(C).
    20. Sarocha Pradyawong & Ankita Juneja & Muhammad Bilal Sadiq & Athapol Noomhorm & Vijay Singh, 2018. "Comparison of Cassava Starch with Corn as a Feedstock for Bioethanol Production," Energies, MDPI, vol. 11(12), pages 1-11, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:11:y:2018:i:4:p:886-:d:140447. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.