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Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis

Author

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  • Rafał Łukajtis

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Piotr Rybarczyk

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Karolina Kucharska

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Donata Konopacka-Łyskawa

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Edyta Słupek

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Katarzyna Wychodnik

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

  • Marian Kamiński

    (Department of Chemical and Process Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland)

Abstract

Pre-treatment is a significant step in the production of second-generation biofuels from waste lignocellulosic materials. Obtaining biofuels as a result of fermentation processes requires appropriate pre-treatment conditions ensuring the highest possible degree of saccharification of the feed material. An influence of the following process parameters were investigated for alkaline pre-treatment of Salix viminalis L.: catalyst concentration (NaOH), temperature, pre-treatment time and granulation. For this purpose, experiments were carried out in accordance to the Box-Behnken design for four factors. In the saccharification process of the pre-treated biomass, cellulolytic enzymes immobilized on diatomaceous earth were used. Based on the obtained results, a mathematical model for the optimal conditions of alkaline pre-treatment prediction is proposed. The optimal conditions of alkaline pre-treatment are established as follows: granulation 0.75 mm, catalyst concentration 7%, pre-treatment time 6 h and temperature 65 °C if the saccharification efficiency and cost analysis are considered. An influence of the optimized pre-treatment on both the chemical composition and structural changes for six various lignocellulosic materials (energetic willow, energetic poplar, beech, triticale, meadow grass, corncobs) was investigated. SEM images of raw and pre-treated biomass samples are included in order to follow the changes in the biomass structure during hydrolysis.

Suggested Citation

  • Rafał Łukajtis & Piotr Rybarczyk & Karolina Kucharska & Donata Konopacka-Łyskawa & Edyta Słupek & Katarzyna Wychodnik & Marian Kamiński, 2018. "Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis," Energies, MDPI, vol. 11(4), pages 1-27, April.
    • Handle: RePEc:gam:jeners:v:11:y:2018:i:4:p:886-:d:140447
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    1. Karolina Kucharska & Patrycja Makoś-Chełstowska & Edyta Słupek & Jacek Gębicki, 2021. "Management of Dark Fermentation Broth via Bio Refining and Photo Fermentation," Energies, MDPI, vol. 14(19), pages 1-16, October.
    2. Mariana Abreu & Luís Silva & Belina Ribeiro & Alice Ferreira & Luís Alves & Susana M. Paixão & Luísa Gouveia & Patrícia Moura & Florbela Carvalheiro & Luís C. Duarte & Ana Luisa Fernando & Alberto Rei, 2022. "Low Indirect Land Use Change (ILUC) Energy Crops to Bioenergy and Biofuels—A Review," Energies, MDPI, vol. 15(12), pages 1-68, June.
    3. Vitor B. Furlong & Luciano J. Corrêa & Roberto C. Giordano & Marcelo P. A. Ribeiro, 2019. "Fuzzy-Enhanced Modeling of Lignocellulosic Biomass Enzymatic Saccharification," Energies, MDPI, vol. 12(11), pages 1-17, June.
    4. Vincent Oriez & Jérôme Peydecastaing & Pierre-Yves Pontalier, 2020. "Lignocellulosic Biomass Mild Alkaline Fractionation and Resulting Extract Purification Processes: Conditions, Yields, and Purities," Clean Technol., MDPI, vol. 2(1), pages 1-25, February.

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