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Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach

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  • Deb, Dipanwita
  • Mallick, Nirupama
  • Bhadoria, P.B.S.

Abstract

The present investigation aims towards simultaneous increase in biomass and carbohydrate accumulation of Anabaena variabilis by formulating an optimal growth condition. Based on the individual factor studies, concentrations of three variables were optimized. An initial pH 8.9, 168.8 mg L−1 MgSO4, and 64.3 mg L−1 NaHCO3 were the best combination resulting in respective ∼1.5 and ∼2.4-fold higher biomass and total carbohydrate yield compared to BG-11-grown control. Carbohydrate profiling indicated ∼2.6-fold rise in the yield of reducing sugar and glycogen. The bioethanol yield was, therefore, enhanced by ∼2.5-fold, reflecting almost two-times higher the value obtained earlier using the biphasic approach. The optimized condition also heightened the yield of the co-products C-phycocyanin and sodium copper chlorophyllin by ∼3-fold, and poly-β-hydroxybutyrate and exopolysaccharides by ∼2.6 and ∼1.8-fold. A ∼60% greater earning was projected from both bioethanol and co-products using the optimal medium under the large-scale set-up compared to control. Thus the present work engineered a robust way to maximize bioethanol production within a shortened time spell, ruling out the constraints of nutrient starvation under biphasic strategies alongside economizing the production process incorporating cyanobacterial refinery approach. Additionally, the details on photobioreactor prospects and sustainability assessment using ‘Water footprint’ would be beneficial for future research.

Suggested Citation

  • Deb, Dipanwita & Mallick, Nirupama & Bhadoria, P.B.S., 2021. "Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach," Renewable Energy, Elsevier, vol. 163(C), pages 1786-1801.
  • Handle: RePEc:eee:renene:v:163:y:2021:i:c:p:1786-1801
    DOI: 10.1016/j.renene.2020.10.086
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    1. Kumar, Anup & Guria, Chandan & Pathak, Akhilendra K., 2018. "Optimal cultivation towards enhanced algae-biomass and lipid production using Dunaliella tertiolecta for biofuel application and potential CO2 bio-fixation: Effect of nitrogen deficient fertilizer, li," Energy, Elsevier, vol. 148(C), pages 1069-1086.
    2. Banerjee, Avik & Guria, Chandan & Maiti, Subodh K., 2016. "Fertilizer assisted optimal cultivation of microalgae using response surface method and genetic algorithm for biofuel feedstock," Energy, Elsevier, vol. 115(P1), pages 1272-1290.
    3. Singh, Harshita & Varanasi, Jhansi L. & Banerjee, Srijoni & Das, Debabrata, 2019. "Production of carbohydrate enrich microalgal biomass as a bioenergy feedstock," Energy, Elsevier, vol. 188(C).
    4. Giorgos Markou & Irini Angelidaki & Elias Nerantzis & Dimitris Georgakakis, 2013. "Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) p latensis," Energies, MDPI, vol. 6(8), pages 1-14, August.
    5. Ngamsirisomsakul, Marika & Reungsang, Alissara & Liao, Qiang & Kongkeitkajorn, Mallika Boonmee, 2019. "Enhanced bio-ethanol production from Chlorella sp. biomass by hydrothermal pretreatment and enzymatic hydrolysis," Renewable Energy, Elsevier, vol. 141(C), pages 482-492.
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