IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v156y2020icp1301-1312.html
   My bibliography  Save this article

Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation

Author

Listed:
  • Ezeilo, Uchenna R.
  • Wahab, Roswanira Abdul
  • Mahat, Naji Arafat

Abstract

Increasing energy demands call for sustainable alternative sources. Solid state fermentation (SSF) of raw oil palm frond leaves (OPFL) as the substrate to produce extracellular cellulases and xylanase by a novel Rhizopus oryzae UC2 (GenBank accession no. MF767597) was optimized. Optimum SSF conditions (30 °C, 40% moisture content, 2.0 × 108 spores/g inoculum size) yielded the maximum carboxymethyl cellulase (CMCase) (94.68 U/g), filter paperase (FPase) (25.46 U/g), β-glucosidase (145.47 U/g) and xylanase (213.99 U/g) activities, showing a broad pH range of between 6.0 and 12.0. Proteome analysis of crude enzyme cocktail revealed three β-glucosidases, as well as one endo-β-1,4-glucanase, exoglanase and endo-β-1,4-xylanase each. Activities of the enzyme complex were maximal at an acidic pH and temperature that ranged between pH 3.0–5.0 and 50–60 °C, respectively. In situ hydrolysis of OPFL released various concentrations of sugars viz. glucose (26.74 mg/g), xylose (1.44 mg/g), fructose (50.8 mg/g) and cellobiose (58.31 mg/g). Moreover, CMCase, FPase, β-glucosidase and xylanase exhibited half-lives of 5.13, 1.48, 18.81, 9.23 h when incubated at 60 °C, respectively. Thus, the desirable qualities of R. oryzae UC2 seen here supported its prospective biocatalytic role for timely and safe production of digestible carbohydrates from agro-industrial biomass for the subsequent biotransformation into biofuel.

Suggested Citation

  • Ezeilo, Uchenna R. & Wahab, Roswanira Abdul & Mahat, Naji Arafat, 2020. "Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation," Renewable Energy, Elsevier, vol. 156(C), pages 1301-1312.
    • Handle: RePEc:eee:renene:v:156:y:2020:i:c:p:1301-1312
    DOI: 10.1016/j.renene.2019.11.149
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148119318518
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2019.11.149?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Noraziah Abu Yazid & Raquel Barrena & Dimitrios Komilis & Antoni Sánchez, 2017. "Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review," Sustainability, MDPI, vol. 9(2), pages 1-28, February.
    2. da Silva, Francinaldo Leite & de Oliveira Campos, Alan & dos Santos, Davi Alves & de Oliveira Júnior, Sérgio Dantas & de Araújo Padilha, Carlos Eduardo & de Sousa Junior, Francisco Caninde & de Macedo, 2018. "Pretreatments of Carnauba (Copernicia prunifera) straw residue for production of cellulolytic enzymes by Trichorderma reesei CCT-2768 by solid state fermentation," Renewable Energy, Elsevier, vol. 116(PA), pages 299-308.
    3. Cripwell, Rosemary & Favaro, Lorenzo & Rose, Shaunita H. & Basaglia, Marina & Cagnin, Lorenzo & Casella, Sergio & van Zyl, Willem, 2015. "Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast," Applied Energy, Elsevier, vol. 160(C), pages 610-617.
    4. Thomas, Leya & Parameswaran, Binod & Pandey, Ashok, 2016. "Hydrolysis of pretreated rice straw by an enzyme cocktail comprising acidic xylanase from Aspergillus sp. for bioethanol production," Renewable Energy, Elsevier, vol. 98(C), pages 9-15.
    5. Taherzadeh-Ghahfarokhi, Maryam & Panahi, Reza & Mokhtarani, Babak, 2019. "Optimizing the combination of conventional carbonaceous additives of culture media to produce lignocellulose-degrading enzymes by Trichoderma reesei in solid state fermentation of agricultural residue," Renewable Energy, Elsevier, vol. 131(C), pages 946-955.
    6. Pandey, Ajay Kumar & Edgard, Gnansounou & Negi, Sangeeta, 2016. "Optimization of concomitant production of cellulase and xylanase from Rhizopus oryzae SN5 through EVOP-factorial design technique and application in Sorghum Stover based bioethanol production," Renewable Energy, Elsevier, vol. 98(C), pages 51-56.
    7. Chandra, R. & Takeuchi, H. & Hasegawa, T., 2012. "Methane production from lignocellulosic agricultural crop wastes: A review in context to second generation of biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(3), pages 1462-1476.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Rosen, Yan & Mamane, Hadas & Gerchman, Yoram, 2021. "Immersed ozonation of agro-wastes as an effective pretreatment method in bioethanol production," Renewable Energy, Elsevier, vol. 174(C), pages 382-390.
    2. Mondal, Sourav & Neogi, Swati & Chakraborty, Saikat, 2024. "Optimization of reactor parameters for amplifying synergy in enzymatic co-hydrolysis and microbial co-fermentation of lignocellulosic agro-residues," Renewable Energy, Elsevier, vol. 225(C).
    3. Bhattacharya, Raikamal & Arora, Sidharth & Ghosh, Sanjoy, 2022. "Utilization of waste pine needles for the production of cellulolytic enzymes in a solid state fermentation bioreactor and high calorific value fuel pellets from fermented residue: Towards a biorefiner," Renewable Energy, Elsevier, vol. 195(C), pages 1064-1076.
    4. Poomani, Merlin Sobia & Mariappan, Iyyadurai & Muthan, Krishnaveni & Subramanian, Venkatesh, 2024. "Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol," Renewable Energy, Elsevier, vol. 225(C).

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Mohd Yasin, Nazlina Haiza & Maeda, Toshinari & Hu, Anyi & Yu, Chang-Ping & Wood, Thomas K., 2015. "CO2 sequestration by methanogens in activated sludge for methane production," Applied Energy, Elsevier, vol. 142(C), pages 426-434.
    2. Senghor, A. & Dioh, R.M.N. & Müller, C. & Youm, I., 2017. "Cereal crops for biogas production: A review of possible impact of elevated CO2," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 548-554.
    3. Rodriguez, Cristina & Alaswad, A. & Benyounis, K.Y. & Olabi, A.G., 2017. "Pretreatment techniques used in biogas production from grass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 68(P2), pages 1193-1204.
    4. Malherbe, Sarel J.M. & Cripwell, Rosemary A. & Favaro, Lorenzo & van Zyl, Willem H. & Viljoen-Bloom, Marinda, 2023. "Triticale and sorghum as feedstock for bioethanol production via consolidated bioprocessing," Renewable Energy, Elsevier, vol. 206(C), pages 498-505.
    5. Du, Jing & Qian, Yuting & Xi, Yonglan & Lü, Xiwu, 2019. "Hydrothermal and alkaline thermal pretreatment at mild temperature in solid state for physicochemical properties and biogas production from anaerobic digestion of rice straw," Renewable Energy, Elsevier, vol. 139(C), pages 261-267.
    6. Davor Mance & Siniša Vilke & Borna Debelić, 2020. "Sustainable Governance of Coastal Areas and Tourism Impact on Waste Production: Panel Analysis of Croatian Municipalities," Sustainability, MDPI, vol. 12(18), pages 1-16, September.
    7. Tamang, Phurba & Tyagi, Vinay Kumar & Gunjyal, Neelam & Rahmani, Ali Mohammad & Singh, Rajesh & Kumar, Pradeep & Ahmed, Banafsha & Tyagi, Pooja & Banu, Rajesh & Varjani, Sunita & Kazmi, A.A., 2023. "Free nitrous acid (FNA) pretreatment enhances biomethanation of lignocellulosic agro-waste (wheat straw)," Energy, Elsevier, vol. 264(C).
    8. Musaab O. El-Faroug & Fuwu Yan & Maji Luo & Richard Fiifi Turkson, 2016. "Spark Ignition Engine Combustion, Performance and Emission Products from Hydrous Ethanol and Its Blends with Gasoline," Energies, MDPI, vol. 9(12), pages 1-24, November.
    9. da Silva, Francinaldo Leite & de Oliveira Campos, Alan & dos Santos, Davi Alves & Batista Magalhães, Emilianny Rafaely & de Macedo, Gorete Ribeiro & dos Santos, Everaldo Silvino, 2018. "Valorization of an agroextractive residue—Carnauba straw—for the production of bioethanol by simultaneous saccharification and fermentation (SSF)," Renewable Energy, Elsevier, vol. 127(C), pages 661-669.
    10. Ndayisenga, Fabrice & Yu, Zhisheng & Zheng, Jianzhong & Wang, Bobo & Liang, Hongxia & Phulpoto, Irfan Ali & Habiyakare, Telesphore & Zhou, Dandan, 2021. "Microbial electrohydrogenesis cell and dark fermentation integrated system enhances biohydrogen production from lignocellulosic agricultural wastes: Substrate pretreatment towards optimization," Renewable and Sustainable Energy Reviews, Elsevier, vol. 145(C).
    11. Weronika Kruszelnicka, 2020. "A New Model for Environmental Assessment of the Comminution Process in the Chain of Biomass Energy Processing †," Energies, MDPI, vol. 13(2), pages 1-21, January.
    12. Bhattacharya, Raikamal & Arora, Sidharth & Ghosh, Sanjoy, 2022. "Utilization of waste pine needles for the production of cellulolytic enzymes in a solid state fermentation bioreactor and high calorific value fuel pellets from fermented residue: Towards a biorefiner," Renewable Energy, Elsevier, vol. 195(C), pages 1064-1076.
    13. Bonassa, Gabriela & Schneider, Lara Talita & Canever, Victor Bruno & Cremonez, Paulo André & Frigo, Elisandro Pires & Dieter, Jonathan & Teleken, Joel Gustavo, 2018. "Scenarios and prospects of solid biofuel use in Brazil," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 2365-2378.
    14. Wenyao Jin & Xiaochen Xu & Fenglin Yang, 2018. "Application of Rumen Microorganisms for Enhancing Biogas Production of Corn Straw and Livestock Manure in a Pilot-Scale Anaerobic Digestion System: Performance and Microbial Community Analysis," Energies, MDPI, vol. 11(4), pages 1-17, April.
    15. Kumari, Dolly & Singh, Radhika, 2018. "Pretreatment of lignocellulosic wastes for biofuel production: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 90(C), pages 877-891.
    16. Dhaundiyal, Alok & Toth, Laszlo & Bacskai, Istvan & Atsu, Divine, 2020. "Analysis of pyrolysis reactor for hardwood (Acacia) chips," Renewable Energy, Elsevier, vol. 147(P1), pages 1979-1989.
    17. Ghosh, Shiladitya & Chowdhury, Ranjana & Bhattacharya, Pinaki, 2017. "Sustainability of cereal straws for the fermentative production of second generation biofuels: A review of the efficiency and economics of biochemical pretreatment processes," Applied Energy, Elsevier, vol. 198(C), pages 284-298.
    18. Chandra, R. & Takeuchi, H. & Hasegawa, T. & Kumar, R., 2012. "Improving biodegradability and biogas production of wheat straw substrates using sodium hydroxide and hydrothermal pretreatments," Energy, Elsevier, vol. 43(1), pages 273-282.
    19. Lindmark, Johan & Thorin, Eva & Bel Fdhila, Rebei & Dahlquist, Erik, 2014. "Effects of mixing on the result of anaerobic digestion: Review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 40(C), pages 1030-1047.
    20. Hagos, Kiros & Zong, Jianpeng & Li, Dongxue & Liu, Chang & Lu, Xiaohua, 2017. "Anaerobic co-digestion process for biogas production: Progress, challenges and perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 76(C), pages 1485-1496.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:156:y:2020:i:c:p:1301-1312. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.