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Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol

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  • Poomani, Merlin Sobia
  • Mariappan, Iyyadurai
  • Muthan, Krishnaveni
  • Subramanian, Venkatesh

Abstract

The use of lignocellulosic biomass to produce value-added products, such as fuel ethanol, is a current priority for global biobased economy. The quest to explore novel microbes, such as yeasts, to attain sustainable products for an ever-growing population has become highly in demand. This study might be the first to explore the rumen yeast Pichia kudriavzevii SVMS2019 for cellulase production to efficiently utilize wheat straw as biomass, which can further be converted to bioethanol. The optimization process was carried out on pre-treated wheat straw by employing one factor at a time (OFAT) and response surface methodology (RSM). Wheat straw pre-treated using NaOH alkali pretreatment was characterized with FTIR, XRD, and SEM studies. The findings revealed that the P. kudriavzevii SVMS2019 obtained maximum cellulase activity of 44.064 IU/ml at 37.5 °C, pH 3.5, 120 h, and 1.5% of NaOH pre-treated wheat straw (substrate concentration) through RSM. It exhibited 1.25-fold increased production compared to OFAT. The stability of crude cellulase was observed at 45 °C and pH 6. Further, the hydrolyzed reducing sugars produced under optimal conditions opted for fermentation, with a maximum bioethanol content of 12.13 g/l obtained at 37.5 °C in a 24 h interval.

Suggested Citation

  • Poomani, Merlin Sobia & Mariappan, Iyyadurai & Muthan, Krishnaveni & Subramanian, Venkatesh, 2024. "Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol," Renewable Energy, Elsevier, vol. 225(C).
    • Handle: RePEc:eee:renene:v:225:y:2024:i:c:s0960148124003616
    DOI: 10.1016/j.renene.2024.120296
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    References listed on IDEAS

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    1. Ezeilo, Uchenna R. & Wahab, Roswanira Abdul & Mahat, Naji Arafat, 2020. "Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation," Renewable Energy, Elsevier, vol. 156(C), pages 1301-1312.
    2. Nicoletta Gronchi & Lorenzo Favaro & Lorenzo Cagnin & Silvia Brojanigo & Valentino Pizzocchero & Marina Basaglia & Sergio Casella, 2019. "Novel Yeast Strains for the Efficient Saccharification and Fermentation of Starchy By-Products to Bioethanol," Energies, MDPI, vol. 12(4), pages 1-13, February.
    3. Avchar, Rameshwar & Lanjekar, Vikram & Kshirsagar, Pranav & Dhakephalkar, Prashant K. & Dagar, Sumit Singh & Baghela, Abhishek, 2021. "Buffalo rumen harbours diverse thermotolerant yeasts capable of producing second-generation bioethanol from lignocellulosic biomass," Renewable Energy, Elsevier, vol. 173(C), pages 795-807.
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